Quantitative Analysis of the Area of the Apical Ectodermal Ridge in Chick Appendages Using Image-J.
Quantitative Analysis of the Area of the Apical Ectodermal Ridge in Chick Appendages Using Image-J.
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DOI：
10.29271/jcpsp.2018.06.419
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发表时间：
2018-05
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影响因子：
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通讯作者：
Hamd Binte Shahab Syed;Muhammad Yunus Khan
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文献类型：
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作者：
Hamd Binte Shahab Syed;Muhammad Yunus Khan
目的利用Image-J软件测定苯妥英钠对鸡翅芽顶外胚层脊(AER)的影响。研究设计 一项实验研究。研究地点和持续时间 巴基斯坦内外科医学院 (CPSP) 区域中心解剖学系，伊斯兰堡，2014 年 1 月至 2015 年 1 月。 方法 选择 60 个“埃及法尤米”品种的受精鸡蛋，分为实验组 (B) 和对照组 (A)，每组 30 个鸡蛋。实验组每个鸡蛋单次注射3.5 mg苯妥英钠。对照组注射相同体积的生理盐水。孵育96小时（第4天）后提取发育中的胚胎，并切成5μm厚的组织切片。这些切片用福尔根核和浅绿色染色。采用Image-J计算鸡翅芽顶端外胚层脊的面积并进行统计分析。结果通过Image-J计算，实验组和对照组的顶外胚层脊面积平均值之间的差异在统计学上不显着。结论 根据 Image-J 的定量结果证明，接触苯妥英后，实验雏鸡的顶端外胚层脊面积的变化并不显着。
OBJECTIVE To determine the effect of sodium phenytoin on the apical ectodermal ridges (AER) of chick wing buds by using the software program Image-J. STUDY DESIGN An experimental study. PLACE AND DURATION OF STUDY Department of Anatomy, Regional Center, College of Physicians and Surgeons Pakistan (CPSP), Islamabad, from January 2014 to January 2015. METHODOLOGY Sixty fertilised chicken eggs of 'Egyptian fayoumi' breed were selected and separated into experimental (B) and control (A) groups, each having 30 eggs. A single dose of 3.5 mg sodium phenytoin was injected into each egg of the experimental group. The controls were injected with the same volume of normal saline. Developing embryos were extracted 96 hours (day 4) after incubation and histological sections were cut at 5 μm thickness. These sections were stained with Feulgen Nuclear and Light Green. The area of apical ectodermal ridges of chick wing buds was calculated by employing Image-J and subjected to statistical analysis. RESULTS The difference between the mean values of the area of apical ectodermal ridges of experimental and control groups, as calculated by Image-J, was found to be statistically insignificant. CONCLUSION Change in the area of the apical ectodermal ridges in experimental chicks, following phenytoin exposure, was insignificant as proven on the basis of quantification by Image-J.