Nutritional stress induces exchange of cell material and energetic coupling between bacterial species
Nutritional stress induces exchange of cell material and energetic coupling between bacterial species
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DOI：
10.1038/ncomms7283
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发表时间：
2015-02-01
影响因子：
16.6
通讯作者：
Giudici-Orticoni, Marie-Therese
中科院分区：
文献类型：
--
作者：
Benomar, Saida;Ranava, David;Giudici-Orticoni, Marie-Therese
了解细菌群落的行为对于了解生物地球化学循环和开发环境生物技术至关重要。在这里，我们证明了两个厌氧细菌，丙酮丁醇梭菌（革兰氏阳性）和脱硫弧菌Hildenborough（革兰氏阴性，硫酸盐还原），其中两个合作伙伴之间的物理相互作用诱导紧急属性之间的人工财团的形成。分子和细胞的方法表明，紧密的细胞-细胞相互作用与分子的交换有关，包括蛋白质，这使得一个合作伙伴（脱硫弧菌）的增长，尽管营养物质短缺。这种物理相互作用诱导丙酮酸十字路口的两种编码酶的基因表达的变化，伴随着代谢通量分布的变化，并允许在不需要基因工程的情况下大幅增加氢气产量。由营养物质短缺引起的脱硫弧菌的压力似乎引发了相互作用。
Knowledge of the behaviour of bacterial communities is crucial for understanding biogeochemical cycles and developing environmental biotechnology. Here we demonstrate the formation of an artificial consortium between two anaerobic bacteria, Clostridium acetobutylicum (Gram-positive) and Desulfovibrio vulgaris Hildenborough (Gram-negative, sulfate-reducing) in which physical interactions between the two partners induce emergent properties. Molecular and cellular approaches show that tight cell-cell interactions are associated with an exchange of molecules, including proteins, which allows the growth of one partner (Desulfovibrio vulgaris) in spite of the shortage of nutrients. This physical interaction induces changes in expression of two genes encoding enzymes at the pyruvate crossroads, with concomitant changes in the distribution of metabolic fluxes, and allows a substantial increase in hydrogen production without requiring genetic engineering. The stress induced by the shortage of nutrients of Desulfovibrio vulgaris appears to trigger the interaction.