ISOLATION AND CHARACTERIZATION OF FTSZ ALLELES THAT AFFECT SEPTAL MORPHOLOGY
ISOLATION AND CHARACTERIZATION OF FTSZ ALLELES THAT AFFECT SEPTAL MORPHOLOGY
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DOI：
10.1128/jb.174.16.5414-5423.1992
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发表时间：
1992-08-01
影响因子：
3.2
通讯作者：
LUTKENHAUS, J
中科院分区：
文献类型：
--
作者：
BI, E;LUTKENHAUS, J
FtsZ基因编码一种重要的细胞分裂蛋白，专门定位于分裂细胞的隔膜。在这项研究中，我们研究了ftsZ2(Rsa)突变对细胞生理学的影响。我们发现，这种突变导致了细胞形态的改变，包括微小细胞的形成和平均细胞长度的增加。此外，该突变对细胞裂解有温度依赖性的影响。在这项研究中，我们偶然分离到了一个新的温度敏感型FtsZ突变，该突变由基因5‘端附近的6个密码子插入组成。该突变被命名为ftsZ26(Ts)，在允许的温度下引起了极形态的改变，并在非允许的温度下阻止了细胞的分裂。极性形态的改变是细胞分裂的结果，并与FtsZ环的几何形状改变有关。分离到在非允许温度下引起细胞裂解的ftsZ26(Ts)基因内冷敏感抑制子。这些结果支持FtsZ环决定分裂位置并与隔膜生物合成机制相互作用的假设。
The ftsZ gene encodes an essential cell division protein that specifically localizes to the septum of dividing cells. In this study we characterized the effects of the ftsZ2(Rsa) mutation on cell physiology. We found that this mutation caused an altered cell morphology that included minicell formation and an increased average cell length. In addition, this mutation caused a temperature-dependent effect on cell lysis. During this investigation we fortuitously isolated a novel temperature-sensitive ftsZ mutation that consisted of a 6-codon insertion near the 5' end of the gene. This mutation, designated ftsZ26(Ts), caused an altered polar morphology at the permissive temperature and blocked cell division at the nonpermissive temperature. The altered polar morphology resulted from cell division and correlated with an altered geometry of the FtsZ ring. An intragenic cold-sensitive suppressor of ftsZ26(Ts) that caused cell lysis at the nonpermissive temperature was isolated. These results support the hypothesis that the FtsZ ring determines the division site and interacts with the septal biosynthetic machinery.