Continuous real-time viability assessment of kidneys based on oxygen consumption.
Continuous real-time viability assessment of kidneys based on oxygen consumption.
复制标题
DOI：
10.1016/j.transproceed.2010.05.082
复制
发表时间：
2010-07
影响因子：
0.9
通讯作者：
Papas, K. K.
中科院分区：
文献类型：
--
作者：
Weegman, B. P.;Kirchner, V. A.;Scott, W. E., III;Avgoustiniatos, E. S.;Suszynski, T. M.;Ferrer-Fabrega, J.;Rizzari, M. D.;Kidder, L. S.;Kandaswamy, R.;Sutherland, D. E. R.;Papas, K. K.
目前供体肾脏的体外质量评估仅限于血管阻力测量和组织学分析。移植前评估器官质量的新技术可以进一步改善临床结果，同时扩大已耗尽的死亡供体池。我们建议在移植前测量全器官耗氧量（WOOCR）作为实时评估肾脏质量的一种方法。采用心源性死亡（DCD）模型获得5个猪肾。肾动脉和肾静脉插管，肾脏连接到一个定制的低温机灌注（HMP）系统，该系统配备了一个在线氧合器和光纤氧传感器。在8℃下灌注肾脏，测量灌注参数和氧分压（pO2），计算WOOCR。在没有在线氧合器的情况下，灌注液在动脉入口和静脉出口的pO2在几分钟内下降到接近0。然而，一旦提供足够的氧合，观察到显著的pO2差异，并用于计算WOOCR。WOOCR一直从可能健康的肾脏测量，结果表明它可以用来区分健康和故意受损的器官。定制的HMP系统配备了氧合器和在线氧传感器，可用于WOOCR测量。我们认为，在移植前保存肾脏和其他器官时，WOOCR是一种很有前景的实时质量评估方法。
Current ex vivo quality assessment of donor kidneys is limited to vascular resistance measurements and histological analysis. New techniques for the assessment of organ quality before transplantation may further improve clinical outcomes while expanding the depleted deceased-donor pool. We propose the measurement of whole organ oxygen consumption rate (WOOCR) as a method to assess the quality of kidneys in real time before transplantation. Five porcine kidneys were procured using a donation after cardiac death (DCD) model. The renal artery and renal vein were cannulated and the kidney connected to a custom-made hypothermic machine perfusion (HMP) system equipped with an inline oxygenator and fiber-optic oxygen sensors. Kidneys were perfused at 8°C, and the perfusion parameters and partial oxygen pressures (pO2) were measured to calculate WOOCR. Without an inline oxygenator, the pO2 of the perfusion solution at the arterial inlet and venous outlet diminished to near 0 within minutes. However, once adequate oxygenation was provided, a significant pO2 difference was observed and used to calculate the WOOCR. The WOOCR was consistently measured from presumably healthy kidneys, and results suggest that it can be used to differentiate between healthy and purposely damaged organs. Custom-made HMP systems equipped with an oxygenator and inline oxygen sensors can be applied for WOOCR measurements. We suggest that WOOCR is a promising approach for the real-time quality assessment of kidneys and other organs during preservation before transplantation.
登录
查看更多内容
影响因子：
1.9
作者：
Crane NJ;Pinto PA;Hale D;Gage FA;Tadaki D;Kirk AD;Levin IW;Elster EA
通讯作者：
Elster EA
活性氧/氧化应激导致小鼠短暂缺血损伤后肾脏纤维化恶化
DOI：
10.1152/ajprenal.90735.2008
发表时间：
2009-08-01
影响因子：
4.2
作者：
Kim, Jinu;Seok, Young Mi;Park, Kwon Moo
通讯作者：
Park, Kwon Moo
影响因子：
8.8
作者：
Snoeijs, M. G. J.;Buurman, W. A.;van Heurn, L. W. E.
通讯作者：
van Heurn, L. W. E.
影响因子：
8.9
作者：
Stowe, David F.;Camara, Amadou K. S.;Harder, David R.
通讯作者：
Harder, David R.
影响因子：
0.9
作者：
Papas, KK;Hering, BJ;Avgoustiniatos, ES
通讯作者：
Avgoustiniatos, ES