Recognition of mucin by the adhesin-flagellar system of Pseudomonas aeruginosa.
Recognition of mucin by the adhesin-flagellar system of Pseudomonas aeruginosa.
复制标题
铜绿假单胞菌的粘附素-鞭毛系统对粘蛋白的识别。
DOI：
10.1164/ajrccm/154.4_pt_2.s170
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Ritchings,BW
中科院分区：
文献类型：
--
作者：
Ramphal,R;Arora,SK;Ritchings,BW
铜绿假单胞菌通过涉及粘附素受体系统的特定机制定植于慢性肺病患者的粘液中。几种粘附素与铜绿假单胞菌与细胞的粘附有关，但参与与粘蛋白粘附的主要粘附素的身份尚不清楚。诱变研究表明，铜绿假单胞菌粘附受'poN基因控制，该基因还调节菌毛蛋白合成、鞭毛形成和其他功能。不受RpoN控制的某些鞭毛基因(例如fliF)的诱变也表明粘附和鞭毛基因之间的密切关系并且不一定是'poN对粘附的独立影响。某些早期鞭毛基因的突变体导致粘附和运动性的丧失，而某些晚期基因的突变体，例如鞭毛蛋白基因flie，则失去运动性但保留粘附性。最近的研究表明，运动性和粘附性均受到称为 fleS-R 的双组分调节系统的调节，而 fleS-R 又受到涉及 poN 的级联中的另一个调节器的控制。具有菌毛的 fleR 突变体与粘蛋白的粘附性很差，这明确表明菌毛在与粘蛋白的粘附中不起主要作用。目前尚不清楚粘附素是鞭毛蛋白还是另一种使用鞭毛输出装置进行定位的蛋白质或两者兼而有之。找到'poN控制下的基因可能会为这些问题提供答案。 Ramphar R、Arora SK、Rltchlngs BW。铜绿假单胞菌的粘附素-鞭毛系统对粘蛋白的识别。
Pseudomonas aeruginosa colonizes the mucus of patients with chronic lung diseases by a specific mechanism involving an adhesin-receptor system. Several adhesins have been implicated in the adhesion of P. aeruginosa to cells, but the identity of the principal adhesin (s) involved in adhesion to mucin is unknown. Mutagenesis studies have indicated that P. aeruginosa adhesion is under the control of the'poN gene, which also regulates pilin synthesis, flagellum formation, and other functions. Mutagenesis of certain flagellar genes that are not controlled by RpoN, eg, fliF, also indicates a close relationship between adhesion and flagellar genes and not necessarily an independent effect of'poN on adhesion. Mutants of certain early flagellar genes lead to the loss of both adhesion and motility, whereas mutants of certain late genes, eg, flie, the gene for flagellin, lose motility but retain adhesion. Recent studies indicate that both motility and adhesion are regulated by a two-component regulatory system called fleS-R, which in turn is controlled by another regulator in a cascade that involves' poN. A fleR mutant possessing pili adheres poorly to mucins, definitively showing that pili do not playa major role in adhesion to mucin. It is unclear whether the adhesin is a flagellar protein or another protein that uses the flagellar export apparatus for localization or both. Finding the gene under control of'poN may provide answers to these questions. Ramphal R, Arora SK, Rltchlngs BW. Recognition of mucin by the adhesln-flagellar system of Pseudomonas aeniglnosa.