CpG Oligodeoxynucleotides Activate Innate Immune Response that Suppresses Infectious Bronchitis Virus Replication in Chicken Embryos
CpG Oligodeoxynucleotides Activate Innate Immune Response that Suppresses Infectious Bronchitis Virus Replication in Chicken Embryos
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DOI：
10.1637/8560-121808-reg.1
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发表时间：
2009-06-01
期刊：
影响因子：
1.4
通讯作者：
Mutwiri, George
中科院分区：
文献类型：
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作者：
Dar, Arshud;Potter, Andy;Mutwiri, George
病原体和免疫调节剂，包括合成的寡核苷酸(CpG ODN)对先天性免疫调节的了解，为改善人类和动物对传染病的预防和治疗策略提供了几种新的途径。然而，在这方面，在禽类药物方面做的工作还不多。在本研究中，我们分析了18日龄鸡胚胎注射CpG ODN 2007(B类)后6小时、24小时、48小时和72小时鸡胚胎脾中干扰素、细胞因子和趋化因子mRNA表达的动态变化。我们的数据显示，CpG ODN处理后，干扰素-γ、IL-1β、IL-6和IL-8以及寡腺苷合成酶A的mRNA表达增强。此外，在鸡传染性支气管炎病毒(IBV)攻击前24小时给鸡胚胎注射CpG ODN能够抑制IBV在不同胚胎组织中的繁殖。根据CpG ODN在体内诱导的细胞因子的类型和动力学，推测CpG ODN在体内接种可增强新生雏鸡对病毒感染的抵抗力，CpG ODN可能有助于开发更有效、更安全的禽类疫苗，包括在卵内接种。
The understanding of innate immune modulation by pathogens and immune-modulating agents, including synthetic oligodeoxynucleotides (CpG ODNs), has offered several new approaches to improve prophylactic and therapeutic strategies against infectious diseases in humans and animals. However, in this regard not much work has been done in avian medicine. In the present study, we analyzed the kinetics of interferon (IFN), cytokine, and chemokine mRNA expression in chicken embryonic spleen at 6 hr, 24 hr, 48 hr, and 72 hr after administration of CpG ODN 2007 (B-class) in 18-day-old chicken embryos. Our data showed enhanced expression of IFN-gamma; interleukin (IL)-1 beta, IL-6, and IL-8; and oligoadenyl synthetase A mRNA after CpG ODN administration. In addition, CpG ODN administration to chicken embryos 24 hr before the challenge with infectious bronchitis virus (IBV) was capable of limiting IBV propagation in different embryonic tissues. Based on the kinetics and type of cytokines induced after in ovo administration of CpG ODN, it may be speculated that in ovo administration of CpG ODNs may enhance resistance from viral infection in neonatal chicks and that CpG ODNs may contribute toward the development of more effective and safer poultry vaccines including in ovo vaccines.