ITAM Signaling by Vav Family Rho Guanine Nucleotide Exchange Factors Regulates Interstitial Transit Rates of Neutrophils In Vivo
ITAM Signaling by Vav Family Rho Guanine Nucleotide Exchange Factors Regulates Interstitial Transit Rates of Neutrophils In Vivo
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DOI：
10.1371/journal.pone.0004652
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发表时间：
2009-02-27
期刊：
影响因子：
3.7
通讯作者：
Swat, Wojciech
中科院分区：
文献类型：
--
作者：
Graham, Daniel B.;Zinselmeyer, Bernd H.;Swat, Wojciech
背景：感染后，中性粒细胞迅速从血液中募集到炎症组织中。嗜中性粒细胞的间质迁移对于在微生物生长压倒宿主的防御之前有效捕获和控制快速增殖的微生物至关重要。然而，调节间质迁移的分子机制是不完全understood.Methodology/Principal Findings：在这里，我们使用双光子显微镜（2PM）研究中性粒细胞反应的离散步骤，在皮下感染细菌。我们的研究表明，从ITAM的受体介导的Vav家族Rho GEFs发出的信号控制的速度，但不是方向性，中性粒细胞迁移到网站的细菌infection.Conclusions/Significance：在这里，我们表明，在中性粒细胞迁移到网站的细菌感染，从ITAM的受体发出的信号，专门控制间质中性粒细胞的速度。
Background: In response to infection, neutrophils are quickly recruited from the blood into inflamed tissues. The interstitial migration of neutrophils is crucial for the efficient capture and control of rapidly proliferating microbes before microbial growth can overwhelm the host's defenses. However, the molecular mechanisms that regulate interstitial migration are incompletely understood.Methodology/Principal Findings: Here, we use two-photon microscopy (2PM) to study discrete steps of neutrophil responses during subcutaneous infection with bacteria. Our study demonstrates that signals emanating from ITAM-containing receptors mediated by Vav family Rho GEFs control the velocity, but not the directionality, of neutrophil migration towards sites of bacterial infection.Conclusions/Significance: Here we show that during neutrophil migration towards sites of bacterial infection, signals emanating from ITAM-containing receptors specifically control interstitial neutrophil velocity.