High-throughput screen of protein expression levels induced by cyclooxygenase-2 during influenza a virus infection
High-throughput screen of protein expression levels induced by cyclooxygenase-2 during influenza a virus infection
复制标题
甲型流感病毒感染期间环氧合酶2诱导的蛋白表达水平的高通量筛选
DOI：
10.1016/j.cca.2011.02.028
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发表时间：
2011-05-12
影响因子：
5
通讯作者：
Zhu, Ying
中科院分区：
文献类型：
--
作者：
Liu, Li;Li, Rui;Zhu, Ying
Background: Detection of proteins productions and the functions during the influenza A virus infection. especially the proteins expression levels in the patients serum are emphasized in the research of host immune response to influenza virus infection. Protein microarray technology provides a high-throughput platform for efficient profiling of protein expression.Methods: We investigated the expression levels of 507 immune-related proteins in the 85 serum of patients infected by influenza A virus H3N2 using an antibody array and validated these findings by infecting peripheral blood mononuclear cells (PBMCs) with A/HongKong/498/97 (H3N2) in vitro. Then we used selective inhibitor of proinflammatory factor cyclooxygenase-2 (COX-2) NS-398 to identify those immunomodulatory proteins regulated by the proinflammatory factor.Results: In patients' serum, the expression levels of 138 proteins changed >2-fold in response to viral infection, including 102 that were upregulated and 36 that were downregulated. One-hundred six proteins were confirmed in PBMCs infected by H3N2. Of the 106 proteins involved in the immune response to influenza virus infection, 48 were regulated by COX-2.Conclusions: Our findings identify the host proteins whose expression levels change in response to influenza virus infection and those involved in the proinflammatory factor COX-2-mediated inflammatory cascade. (C) 2011 Elsevier B.V. All rights reserved.
