Maintenance of the synthesis of alpha B-crystallin and progressive expression of beta Bp-crystallin in human fetal lens epithelial cells in culture.
Maintenance of the synthesis of alpha B-crystallin and progressive expression of beta Bp-crystallin in human fetal lens epithelial cells in culture.
复制标题
在培养的人胎儿晶状体上皮细胞中维持 α B-晶状体蛋白的合成和 β Bp-晶状体蛋白的渐进表达。
DOI：
10.1016/0012-1606(88)90446-0
复制
发表时间：
1988
影响因子：
2.7
通讯作者：
Bhat,SP
中科院分区：
文献类型：
--
作者：
Nagineni,CN;Bhat,SP
We have cultured and maintained human fetal lens epithelial cells for several months in primary, secondary, and tertiary culture(s). These cells show unabated synthesis of αB-crystallin (αB), a lens epithelial cell-specific marker, and progressive expression of βBp-crystallin (βBp), a major polypeptide of the differentiated lens fiber cellsin vivo. Interestingly, the expression of βBp was found to be dependent on subculturing of the cells and not on the age of cultures. These observations demonstrate that human fetal lens epithelial cells can be culturedin vitrowithout the loss of lens specific characteristics and with commitment to differentiation at the biochemical level.
