The live cell irradiation and observation setup at SNAKE
The live cell irradiation and observation setup at SNAKE
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DOI：
10.1016/j.nimb.2009.03.071
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发表时间：
2009-06-15
影响因子：
1.3
通讯作者：
Dollinger, G.
中科院分区：
文献类型：
--
作者：
Hable, V.;Greubel, C.;Dollinger, G.
我们描述了一个新的设置在离子微探针蛇（超导纳米应用核（克恩-）物理实验）在慕尼黑14 MV串联加速器，有利于活细胞照射与亚微米分辨率和在线光学成像的细胞之前和之后的最先进的相差和荧光显微镜照射。将细胞保持在37 ℃的标准细胞生长条件下的细胞培养基中。照射后，可以在0.5秒内从单离子照射条件切换到细胞观察。第一个实验是针对细胞核的亚结构进行的，所述亚结构由通过55 MeV单碳离子照射掺入细胞DNA中的TexasRed标记的核苷酸标记。此外，我们显示了第一个在线序列的短时间动力学的Mdc 1蛋白积累在附近的双链断裂后，碳离子照射。(C)2009爱思唯尔有限公司版权所有。
We describe a new setup at the ion microprobe SNAKE (Superconducting Nanoscope for Applied nuclear (Kern-) physics Experiments) at the Munich 14 MV Tandem accelerator that facilitates both living cell irradiation with sub micrometer resolution and online optical imaging of the cells before and after irradiation by state of the art phase contrast and fluorescence microscopy. The cells are kept at standard cell growth conditions at 37 degrees C in cell culture medium. After irradiation it is possible to switch from single ion irradiation conditions to cell observation within 0.5 s. First experiments were performed targeting substructures of a cell nucleus that were tagged by TexasRed labeled nucleotides incorporated in the cellular DNA by 55 MeV single carbon ion irradiation. In addition we show first online sequences of short time kinetics of Mdc1 protein accumulation in the vicinity of double strand breaks after carbon ion irradiation. (C) 2009 Elsevier B.V. All rights reserved.