Use of Poisons in Determination of Microbial Manganese Binding Rates in Seawater
Use of Poisons in Determination of Microbial Manganese Binding Rates in Seawater
复制标题
使用毒物测定海水中微生物锰结合率
DOI：
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发表时间：
1984
影响因子：
4.4
通讯作者：
K. Nealson
中科院分区：
文献类型：
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作者：
R. Rosson;B. Tebo;K. Nealson
建立了一种确定微生物是否参与海洋环境中锰(II)沉淀的方法。用放射性~(54)Mn(II)作示踪剂，研究了在生物毒物存在和不存在的情况下，Mn(II)[即捕获在0.2-μm膜滤膜上的~(54)Mn(II)]的沉淀(结合和氧化)。在实验室对照实验中测试了各种抗生素、固定剂和代谢抑制剂，以选择不干扰锰化学的毒物。如果(I)它们对Mn(II)的络合作用不比离子交换树脂CHILEX-100强，(Ii)它们不干扰合成的δ-MnO_2(锰酸盐)对54Mn(II)的吸附，(Iii)它们不会使预吸附在合成锰酸盐上的54Mn(II)解吸，(Iv)它们不会增溶合成的54MnO_2，则这些毒物被认为是合适的。此外，还测试了几种已知的螯合剂、还原剂和缓冲液，它们通常添加到微生物生长介质中或用于生化分析。大多数添加剂在一定程度上干扰了锰的化学作用。然而，至少有一种抑制剂，叠氮化钠，或叠氮化钠、青霉素和四环素的混合物被证明适合用于54Mn(II)结合的现场研究。甲醛也可以在短时间内(1-3小时)使用，但不适合进行较长时间的研究。将该方法应用于加拿大不列颠哥伦比亚省萨尼奇湾的降水研究。研究表明，细菌能显着提高富锰颗粒层中锰(II)的去除速率，该颗粒层恰好位于水柱中的氧-硫化氢界面上方。
A method was developed to determine whether microorganisms mediate the precipitation of manganese(II) in the marine environment. Radioactive 54Mn(II) was used as a tracer to measure the precipitation (binding and oxidation) of Mn(II) [i.e., the 54Mn(II) trapped on 0.2-μm membrane filters] in the presence and absence of biological poisons. A variety of antibiotics, fixatives, and metabolic inhibitors were tested in laboratory control experiments to select poisons that did not interfere in the chemistry of manganese. The poisons were deemed suitable if (i) they did not complex Mn(II) more strongly than the ion-exchange resin Chelex 100, (ii) they did not interfere in the adsorption of 54Mn(II) onto synthetic δMnO2 (manganate), (iii) they did not cause desorption of 54Mn(II) which had been preadsorbed onto synthetic manganate, and (iv) they did not solubilize synthetic 54manganate. In addition, several known chelators, reducing agents, and buffers normally added to microbiological growth media or used in biochemical assays were tested. Most additions interfered to some extent with manganese chemistry. However, at least one inhibitor, sodium azide, or a mixture of sodium azide, penicillin, and tetracycline was shown to be appropriate for use in field studies of 54Mn(II) binding. Formaldehyde could also be used in short incubations (1 to 3 h) but was not suitable for longer time course studies. The method was applied to studies of Mn(II) precipitation in Saanich Inlet, British Columbia, Canada. Bacteria were shown to significantly enhance the rate of Mn(II) removal from solution in the manganese-rich particulate layer which occurs just above the oxygen-hydrogen sulfide interface in the water column.