Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization
Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization
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DOI：
10.1016/j.fertnstert.2008.11.028
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发表时间：
2010-03-01
影响因子：
6.7
通讯作者：
Yoshimura, Yasunori
中科院分区：
文献类型：
--
作者：
Mizusawa, Yuri;Kuji, Naoaki;Yoshimura, Yasunori
目的：研究卵母细胞特异性连接历史蛋白（hH 1FOO）在人卵巢中的表达及其在卵胞浆内单精子注射（ICSI）后掺入精子染色质的情况。设计：实验室研究。地点：大学医院。患者：从卵巢切除术患者中获得人卵巢组织。从接受IVF和ICSI.Intervention的不孕患者中获得人卵母细胞和胚胎：使用靶向预测的hH 1FOO蛋白的多克隆兔抗体进行免疫组织化学分析。结果：hH 1FOO蛋白在从原始卵泡到Graafian卵泡的所有卵母细胞中均有表达。在ICSI后未受精的卵母细胞中，45.5%的卵母细胞在没有hH 1FOO掺入的情况下注射精子的染色质浓缩，9%的卵母细胞在hH 1FOO掺入的情况下浓缩，45.5%的卵母细胞在hH 1FOO掺入的情况下去浓缩。结论：hR 1FOO蛋白在人原始卵泡至胚胎发育早期的卵母细胞中均有表达。ICSI后仍浓缩的精子核可通过hH 1FOO掺入分为两类，这可能提供有关受精失败的有价值的信息。（Fertil Steril（R）2010;93：11，34 -41.（C）2010年由美国生殖医学学会。
Objective: To investigate the expression of oocyte-specific linker historic protein (hH1FOO) inhuman ovaries and its incorporation into sperm chromatin after intracytoplasmic sperm injection (ICSI).Design: Laboratory study.Setting: University hospital.Patient(s): Human ovarian tissues were obtained from patients at oophorectomy. Human oocytes and embryos were obtained from infertile patients undergoing IVF and ICSI.Intervention(s): A polyclonal rabbit antibody targeting the predicted hH1FOO protein was used for immunohistochemical analysis. Western blot analysis and the reverse transcriptase-nested polymerase chain reaction were done to detect hH1FOO in chromatin of germinal vesicle-stage oocytes through to two-cell embryos.Main Outcome Measure(s): The hH1FOO antibody reactivity of oocytes, ovarian tissues, and sperm chromatin after ICSI.Result(s): hH1FOO protein was localized in all oocytes from primordial to Graafian follicles. In unfertilized oocytes after ICSI, the chromatin of injected sperm was condensed without hH1FOO incorporation in 45.5% of oocytes, condensed with hH1FOO incorporation in 9%, and decondensed with hH1FOO incorporation in 45.5%. None of the oocytes contained decondensed sperm chromatin without hHFOO incorporation.Conclusion(s): hR1FOO protein was expressed by human oocytes from primordial follicles to early embryogenesis. Sperm nuclei that were still condensed after ICSI could be separated into two categories by hH1FOO incorporation, which might provide valuable information regarding failed fertilization. (Fertil Steril (R) 2010;93:11,34-41. (C)2010 by American Society for Reproductive Medicine.)