Surface modification of PE porous membranes based on the strong adhesion of polydopamine and covalent immobilization of heparin
Surface modification of PE porous membranes based on the strong adhesion of polydopamine and covalent immobilization of heparin
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基于聚多巴胺强粘附和肝素共价固定的PE多孔膜表面改性
DOI：
10.1016/j.memsci.2010.08.017
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发表时间：
2010-11-15
影响因子：
9.5
通讯作者：
Zhu, Bao-Ku
中科院分区：
文献类型：
--
作者：
Jiang, Jin-Hong;Zhu, Li-Ping;Zhu, Bao-Ku
基于多巴胺在潮湿条件下的自聚合和强粘附特性，将疏水聚乙烯（PE）多孔膜浸泡在多巴胺水溶液中24小时，对其进行表面修饰。随后，通过肝素与反应性聚多巴胺层的偶联，将肝素共价固定在膜上。利用衰减全反射傅里叶变换红外（ATR-FTIR）光谱和x射线光电子能谱（XPS）对膜表面的化学成分进行了测定，证实了聚多巴胺的成功引入和肝素分子的固定化。采用扫描电镜（SEM）和原子力显微镜（AFM）研究了表面改性后表面形貌的变化。水接触角测量结果表明，聚多巴胺包被和肝素固定后，PE膜的亲水性明显提高。体外血液相容性试验结果证明，表面肝素化可显著抑制血小板粘附，增强PE膜抗凝能力。这项工作为提高惰性PE多孔膜的渗透性和生物相容性提供了一种方便的方法，可用于生物医学和血液接触应用。(C) 2010 Elsevier B.V.版权所有
Based on the self-polymerization and strong adhesion characteristic of dopamine in wet conditions, the hydrophobic polyethylene (PE) porous membranes were surface-modified via simply immersing them into dopamine aqueous solution for 24 h. Subsequently, heparin was immobilized covalently onto the resultant membrane by the coupling between heparin and reactive polydopamine layer. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) were utilized to determine the chemical compositions of membrane surface, which confirmed the successful introduction of polydopamine and immobilization of heparin molecules. Scanning electronic microscopy (SEM) and atomic force microscopy (AFM) were employed to investigate the changes in surface morphologies after surface modification. The data of water contact angle measurements indicated that the hydrophilicity of PE membranes was remarkably improved after polydopamine coating and heparin immobilization. The results of in vitro hemocompatibility test proved that surface heparinization significantly suppressed the adhesion of platelet and enhanced the anticoagulation ability of PE membranes. This work offered a convenient approach to improve the permeability and biocompatibility of inert PE porous membranes for their biomedical and blood-contacting applications. (C) 2010 Elsevier B.V. All rights reserved.