The expression level of C19MC miRNAs in early pregnancy and in response to viral infection
The expression level of C19MC miRNAs in early pregnancy and in response to viral infection
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DOI：
10.1016/j.placenta.2017.03.011
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发表时间：
2017-05-01
期刊：
影响因子：
3.8
通讯作者：
Sadovsky, Yoel
中科院分区：
文献类型：
--
作者：
Dumont, Tina M. F.;Mouillet, Jean-Francois;Sadovsky, Yoel
简介：我们以前已经表明，从染色体19 miRNA簇（C19 MC）产生的miRNA，几乎只在灵长类动物滋养层中表达，并释放到母体循环中，减少非胎盘细胞中的病毒复制，并可以调节绒毛外滋养层的迁移行为。我们试图定义C19 MC miRNA在妊娠早期的表达模式，并在体外和体内的病毒infection in vitro and in vivo.Methods：我们前瞻性地跟踪接受体外受精（IVF）的妇女，并使用RT-qPCR测定其血液中C19 MC miRNA的水平。为了检测病毒暴露对C19 MC miRNA表达的影响，我们使用了三种系统：（1）过表达C19 MC簇并在妊娠期间暴露于披膜病毒科的转基因小鼠，（2）体外暴露于水泡性口炎病毒的培养的原代人滋养层细胞，以及（3）妊娠期间暴露于巨细胞病毒的妇女的羊水。在27例IVF妊娠中，早在植入后2周就检测到C19 MC miRNAs，此后其水平增加。小鼠胎盘中C19 MC miRNA表达水平对病毒暴露的反应没有变化。类似地，水泡性口炎病毒感染原代人滋养层细胞并没有选择性地增加C19 MC miRNA的表达。C19 MC miRNA在羊水中的表达不受垂直传播的cytomegalovirus.Discussion的影响：C19 MC miRNA在母体循环中的表达非常早期在怀孕表明在建立母胎界面的作用。C19 MC miRNA的水平不受不同类型病毒感染的影响。(C)2017爱思唯尔有限公司版权所有
Introduction: We have previously shown that miRNAs produced from the Chromosome 19 MiRNA Cluster (C19MC), which are expressed almost exclusively in primate trophoblasts and are released into the maternal circulation, reduce viral replication in non-placental cells and can modulate migratory behavior of extravillous trophoblast. We sought to define the expression pattern of C19MC miRNA in early pregnancy and in response to viral infection in vitro and in vivo.Methods: We prospectively followed women undergoing in vitro fertilization (IVF) and determined their blood level of C19MC miRNA using RT-qPCR. To examine the effect of viral exposure on C19MC miRNAs expression, we used three systems: (1) a transgenic mouse overexpressing the C19MC cluster and exposed to Togaviridae during pregnancy, (2) cultured primary human trophoblasts exposed to Vesicular Stomatitis Virus in vitro, and (3) amniotic fluid from women exposed to cytomegalovirus during pregnancy.Results: In 27 IVF pregnancies, C19MC miRNAs were detected as early as 2 weeks after implantation, and their levels increased thereafter. There was no change in C19MC miRNA expression levels in the mouse placenta in response to viral exposure. Similarly, Vesicular Stomatitis Virus infection of primary human trophoblast did not selectively increase C19MC miRNA expression. C19MC miRNA expression in the amniotic fluid was not affected by vertical transmission of cytomegalovirus.Discussion: The expression of C19MC miRNAs in maternal circulation very early in pregnancy suggests a role in the establishment of the maternal-fetal interface. The levels of C19MC miRNA are not influenced by diverse types of viral infection. (C) 2017 Elsevier Ltd. All rights reserved.