THE DIRECTION OF MEMBRANE LIPID FLOW IN LOCOMOTING POLYMORPHONUCLEAR LEUKOCYTES
THE DIRECTION OF MEMBRANE LIPID FLOW IN LOCOMOTING POLYMORPHONUCLEAR LEUKOCYTES
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DOI：
10.1126/science.2315695
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发表时间：
1990-03-09
期刊：
影响因子：
56.9
通讯作者：
JACOBSON, K
中科院分区：
文献类型：
--
作者：
LEE, J;GUSTAFSSON, M;JACOBSON, K
本研究的目的是确定膜脂流动的方向，在迁移细胞。人多形核白细胞的质膜用荧光脂质类似物二十六烷酰吲哚羰花青染色。在细胞表面上垂直于细胞运动方向的线被光漂白。微光荧光显微镜和数字图像处理技术被用来分析一系列的图像，在短时间内采取的光漂白后。 漂白的线在通过扩散恢复擦除之前保持可见约5秒。荧光强度分布的检查允许线和细胞运动的速度之间进行比较。结果表明，在运动过程中，漂白线以与细胞相同的速度向前移动，反驳了逆行脂质流动模型的运动。相反，质膜脂质似乎根据膜的单位运动或运动的坦克轨道模型向前移动。
The objective of this study was to determine the direction of membrane lipid flow in locomoting cells. The plasma membrane of human polymorphonuclear leukocytes was stained with a fluorescent lipid analog dihexadecanoyl indocarbocyanine. A line was photobleached on the cell surface perpendicular to the direction of cell motion. Low-light-level fluorescence microscopy and digital image-processing techniques were used to analyze a series of images taken at short intervals after photobleaching. The bleached line remained visible for about 5 seconds before being erased by diffusional recovery. Examination of fluorescence intensity profiles allowed a comparison to be made between the velocities of line and cell movement. Results indicate that the bleached line moves forward with the same velocity as the cell during locomotion, refuting the retrograde lipid flow model of locomotion. Instead, the plasma membrane lipid appears to move forward according to either the unit movement of membrane or the tank track model of locomotion.