A mammalian host-vector system that regulates expression and amplification of transfected genes by temperature induction.
A mammalian host-vector system that regulates expression and amplification of transfected genes by temperature induction.
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一种哺乳动物宿主载体系统，通过温度诱导调节转染基因的表达和扩增。
DOI：
10.1126/science.2981116
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发表时间：
1985
期刊：
影响因子：
56.9
通讯作者：
R. Tjian
中科院分区：
文献类型：
--
作者：
D. Rio;S. G. Clark;R. Tjian
分离并表征了允许温度依赖性调节载体 DNA 复制的 SV40 转化猿细胞。这些细胞系（ts COS 细胞）在劳斯肉瘤病毒长末端重复的转录控制下产生高水平的耐热大 T 抗原。 ts COS细胞系可以补充SV40 A基因突变体，并支持含有SV40起源的载体在33摄氏度下复制，但不能在40摄氏度下复制。现在应该可以调节转染质粒DNA的拷贝数，并通过调节ts COS细胞中的T抗原活性，以整合DNA或自主复制附加体的形式维持可选择的载体序列。
SV40-transformed simian cells that permit temperature-dependent regulation of vector DNA replication were isolated and characterized. These cell lines (ts COS cells) produce high levels of thermolabile large T antigen under the transcriptional control of the Rous sarcoma virus long terminal repeat. The ts COS cell lines can complement SV40 A gene mutants and support replication of SV40-origin containing vectors at 33 degrees C but not at 40 degrees C. It should now be possible to regulate the copy number of transfected plasmid DNA's and also maintain selectable vector sequences either as integrated DNA or as autonomously replicating episomes by modulating T antigen activity in ts COS cells.