Using FM Dyes to Monitor Clathrin-Mediated Endocytosis in Primary Neuronal Culture
Using FM Dyes to Monitor Clathrin-Mediated Endocytosis in Primary Neuronal Culture
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DOI：
10.1007/978-1-4939-8719-1_18
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发表时间：
2018-01-01
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影响因子：
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通讯作者：
Smillie, Karen J.
中科院分区：
文献类型：
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作者：
Cousin, Michael A.;Gordon, Sarah L.;Smillie, Karen J.
该方案利用亲脂性FM染料来真实的实时监测膜再循环。FM染料在溶液中几乎是无荧光的，但当膜结合时是强烈荧光的，结合不同发射波长的灵活性，使这些染料成为研究网格蛋白介导的内吞作用的绝佳选择，以及其他膜运输和再循环途径。
This protocol utilizes lipophilic FM dyes to monitor membrane recycling in real time. FM dyes are virtually nonfluorescent in solution but when membrane bound are intensely fluorescent, combined with the flexibility of different emission wavelengths make these dyes an excellent choice for investigating clathrin-mediated endocytosis, among other membrane trafficking and recycling pathways.