Specific binding of the mononuclear phagocyte colony-stimulating factor CSF-1 to the product of the v-fms oncogene.
Specific binding of the mononuclear phagocyte colony-stimulating factor CSF-1 to the product of the v-fms oncogene.
复制标题
单核吞噬细胞集落刺激因子 CSF-1 与 v-fms 癌基因产物的特异性结合。
DOI：
10.1073/pnas.83.10.3331
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发表时间：
1986
影响因子：
11.1
通讯作者：
Rettenmier,CW
中科院分区：
文献类型：
--
作者：
Sacca,R;Stanley,ER;Sherr,CJ;Rettenmier,CW
由猫肉瘤病毒（SM-FeSV）的麦克多诺株转化的细胞表达v-fms编码的糖蛋白，其在细胞表面的表达与转化的表型相关。小鼠单核吞噬细胞生长因子CSF-1特异性结合SM-FeSV转化细胞的高亲和力位点与正常猫巨噬细胞上检测到的位点无法区分。针对v-fms编码的表位的单克隆抗体竞争CSF-1与SM-FeSV转化的细胞的结合，并且化学交联证明鼠CSF-1与细胞表面的v-fms基因产物结合。虽然发现SM-FeSV转化的成纤维细胞系分泌CSF-1，但转化细胞的生长不受v-fms基因产物或生长因子抗体的影响。在没有CSF-1的情况下观察到膜中v-fms产物的酪氨酸磷酸化，并且通过添加鼠生长因子没有增强。这些数据支持c-fms原癌基因产物与CSF-1受体相关且可能相同的假设，并表明v-fms编码的激酶在不存在外源性生长因子的情况下起作用。
Cells transformed by the McDonough strain of feline sarcoma virus (SM-FeSV) express a v-fms-encoded glycoprotein whose expression at the cell surface correlates with the transformed phenotype. The mouse mononuclear phagocyte growth factor CSF-1 specifically binds to SM-FeSV-transformed cells at high-affinity sites indistinguishable from those detected on normal feline macrophages. A monoclonal antibody to a v-fms-encoded epitope competed for CSF-1 binding to SM-FeSV-transformed cells, and chemical crosslinking demonstrated that murine CSF-1 bound to the v-fms gene product at the cell surface. Although SM-FeSV-transformed fibroblast lines were found to secrete CSF-1, the growth of transformed cells was not affected by antibodies to the v-fms gene product or to the growth factor. Tyrosine phosphorylation of the v-fms products in membranes was observed in the absence of CSF-1 and was not enhanced by addition of the murine growth factor. The data support the hypothesis that the c-fms protooncogene product is related, and possibly identical, to the CSF-1 receptor and suggest that the v-fms-encoded kinase functions in the absence of an exogenous growth factor.