A kinetic model for growth and biosynthesis of medium-chain-length poly-(3-hydroxyalkanoates) in Pseudomonas putida
A kinetic model for growth and biosynthesis of medium-chain-length poly-(3-hydroxyalkanoates) in Pseudomonas putida
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DOI：
10.1590/s0104-66322008000200001
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发表时间：
2008-06-01
影响因子：
1.2
通讯作者：
Ramachandran, K. B.
中科院分区：
文献类型：
--
作者：
Annuar, M. S. M.;Tan, I. K. P.;Ramachandran, K. B.
以皂化棕榈仁油为碳源，铵为限制性营养物质，建立了恶臭假单胞菌PGA 1分批培养的动力学模型。微生物的生长是很好地描述使用。Tessier型模型考虑了高浓度铵的抑制作用。细胞的氨消耗速率与生长速率不成比例。恶臭假单胞菌PGA 1细胞的中链长聚（3-羟基链烷酸酯）（PHA（MCL））的细胞内生产通过修改的Luedeking-Piret动力学合理地建模，其中纳入了铵对产物合成抑制（或减少）的功能高于阈值水平。
A kinetic model is presented giving a mathematical description of batch culture of Pseudomonas putida PGA1 grown using saponified palm kernel oil as carbon source and ammonium as the limiting nutrient. The growth of the micro-organism is well-described using. Tessier-type model which takes into account the inhibitory effect of ammonium at high concentrations. The ammonium consumption rate by the cells is related ill proportion to the rate of growth. The intracellular production of medium-chain-length poly(3-hydroxyalkanoates) (PHA(MCL)) by P. putida PGA1 cells is reasonably modeled by the modified Luedeking-Piret kinetics, which incorporate a function of product Synthesis inhibition (or reduction) by ammonium above a threshold level.