Behcet's Disease with Active Uveitis: Detection of Serum Protein Biomarkers Using MALDI‐TOF‐MS
Behcet's Disease with Active Uveitis: Detection of Serum Protein Biomarkers Using MALDI‐TOF‐MS
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DOI：
10.1002/ar.22502
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发表时间：
2012-07
期刊：
影响因子：
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通讯作者：
Xin Wang;Meiying Zhang;Jing Xie;Zhi Li;P. Wang
中科院分区：
文献类型：
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作者：
Xin Wang;Meiying Zhang;Jing Xie;Zhi Li;P. Wang
白塞病(BD)是一种缺乏敏感和特异诊断方法的多系统自身免疫性疾病。本研究的目的是确定BD的潜在生物标志物，并建立诊断模型。将BD、Vogt-Koyanagi-Harada综合征(VKH)患者和健康对照组(HC)的血清样本随机分为训练组(49例，VKH组，31例，HC组)和试验组(13例，VKH组，10例，HC组)。用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)获得蛋白质组质谱。用1,644、1,711、2,023、4,347、6,628和8,559处的m/z峰构建了诊断BD的模型。该诊断模型对BD和非BD对照的敏感性为83.67%(41/49)，特异性为89.87%(71/79)。本组盲法检测BD的灵敏度和特异度分别为84.6%和90.48%。结果提示，磁珠与MALDI-TOF-MS相结合的蛋白质组指纹技术在BD的诊断中具有潜在的应用价值。生物标记物分类模型适用于BD的初步鉴定，可能成为BD诊断和鉴别诊断的有用工具。Anat Rec，2012年。©2012 Wiley期刊，Inc.
Behcet's Disease (BD) is a multisystem autoimmune disorder that lacks sensitive and specific diagnostic methods. The aim of this study was to identify potential biomarkers specific for BD and to establish a diagnostic model. Serum samples from patients with BD, Vogt‐Koyanagi‐Harada syndrome (VKH), and healthy controls (HC) were randomly divided into a training set (49 BD, 31 VKH, and 48 HC) and a testing set (13 BD, 10 VKH, and 11 HC). Proteomic mass spectra were generated by matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS). Thirty‐nine differential m/z peaks associated with BD were identified, and the m/z peaks at 1,644, 1,711, 2,023, 4,347, 6,628, and 8,559 were used to construct a model for the diagnosis of BD. This diagnostic model can distinguish BD from non‐BD controls with a sensitivity of 83.67% (41/49) and a specificity of 89.87% (71/79). BD was detected in our blinded testing set with good sensitivity and specificity of 84.6 and 90.48%, respectively. The results suggested that proteomic fingerprint technology combining magnetic beads with MALDI‐TOF‐MS has potential for the diagnosis of BD. The biomarker classification model was suitable for preliminary identification of BD and could potentially serve as a useful tool for BD diagnosis and differential diagnosis. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.