Expression of the p53 protein during the cell cycle of human peripheral blood lymphocytes.
Expression of the p53 protein during the cell cycle of human peripheral blood lymphocytes.
复制标题
人外周血淋巴细胞细胞周期中p53蛋白的表达。
DOI：
10.1016/0014-4827(85)90233-2
复制
发表时间：
1985
影响因子：
3.7
通讯作者：
Baserga,R
中科院分区：
文献类型：
--
作者：
Mercer,WE;Baserga,R
在植物血凝素(PHA)刺激的人外周血淋巴细胞(PBL)和单核/巨噬细胞耗竭淋巴细胞(MDL)培养中，我们研究了细胞P53蛋白在诱导细胞大小增长和细胞DNA复制中的作用。我们的结果表明，在暴露于PHA的人淋巴细胞中，P53蛋白合成和积累的诱导与细胞DNA复制的程度有关，而不是与大小的增长有关。此外，P53的诱导依赖于T细胞有丝分裂原--白介素2的存在。抗白细胞介素2受体的单抗(抗Tac)可抑制PHA刺激的细胞DNA合成，这种抑制作用与P53阳性细胞百分率的降低有关。我们的结论是，P53蛋白是一个细胞周期依赖的基因，它的表达可以被不同类型的细胞中的不同有丝分裂原调节。
We have investigated the role of the cellular p53 protein in the induction of growth in size and cell DNA replication in human peripheral blood lymphocytes (PBL) and in monocyte/macrophage-depleted lymphocyte (MDL) cultures stimulated with phytohemagglutinin (PHA). Our results show that in human lymphocytes exposed to PHA, the induction of p53 protein synthesis and accumulation correlates with the extent of cellular DNA replication, rather than with growth in size. Moreover, the induction of p53 is dependent on the presence of the T-cell mitogen, Interleukin-2. A monoclonal antibody to Interleukin-2 receptors (anti-Tac) inhibits PHA-stimulated cellular DNA synthesis, and this inhibition is correlated with a reduction in the percentage of p53-positive cells. We conclude from this work that the p53 protein is a cell cycle-dependent gene whose expression can be regulated by different mitogens in different cell types.