Artificial tissue bioreactor (ATB) for biological and imaging applications.
Artificial tissue bioreactor (ATB) for biological and imaging applications.
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用于生物和成像应用的人工组织生物反应器 (ATB)。
DOI：
10.1109/embc.2012.6346452
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Shoghi,KooreshI
中科院分区：
文献类型：
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作者：
Whitehead,TimothyD;Nemanich,SamuelT;Shoghi,KooreshI
三维（3D）培养系统越来越多地应用于组织生物学研究。在这项工作中，我们报告了一种人工组织生物反应器（ATB）的开发，该反应器旨在模拟体内组织的3D结构和微环境，具有多种采样途径，包括组织室，用于下游分析。此外，ATB与microPET Focus F220集成，用于体内成像应用。作为概念验证，我们利用HepG2细胞表征了脂质对葡萄糖利用的影响。ATB研究采用PPAR-γ激动剂吡格列酮进行治疗前和治疗后干预。同时，葡萄糖耐量试验（GTT）在培养基样品上进行，以评估细胞对葡萄糖的摄取，作为胰岛素信号敏感性的测量。脂肪酸在ATB细胞腔室的摄取是用[11C]棕榈酸酯和显微pet成像来测量的。总体而言，ATB将促进现有和新型放射性药物的使用，以发现验证并将从ATB研究中获得的见解转化为临床前动物研究和临床评估。
Three-dimensional (3D) culture systems are increasingly applied to study tissue biology. In this work, we report on the development of an artificial tissue bioreactor (ATB) designed to simulate the 3D structure and microenvironment of tissues in vivo, with multiple avenues of sampling, including the tissue chamber, for downstream analysis. Additionally, the ATB is integrated with the microPET Focus F220 for in-vivo imaging applications. As a proof-of-concept, we characterized the effects of lipids on glucose utilization using HepG2 cells. ATB studies were performed pre- and post- therapeutic intervention with the PPAR-γ agonist pioglitazone. In parallel, Glucose Tolerance Test (GTT) is performed on media samples to assess glucose uptake by cells as a measures of insulin signaling sensitivity. Fatty acid uptake in the ATB cell chamber is measured using [11C]Palmitate with microPET imaging. Overall, the ATB will facilitate the use of existing and novel radiopharmaceuticals in discovery of validating and translating insights derived from ATB studies to pre-clinical animal studies, to clinical evaluation.