Copper Intoxication; Antioxidant Defenses and Oxidative Damage in Rat Brain
Copper Intoxication; Antioxidant Defenses and Oxidative Damage in Rat Brain
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DOI：
10.1007/s12011-008-8219-3
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发表时间：
2009-01-01
影响因子：
3.9
通讯作者：
Uzun, Hafize
中科院分区：
文献类型：
--
作者：
Ozcelik, Dervis;Uzun, Hafize
铜（Cu）是参与许多重要生物过程的许多重要酶的组成部分。尽管铜对生命至关重要，但当组织浓度升高时，它会对细胞产生毒性。近年来的研究报道了铜对多种组织的氧化损伤。在本研究中，我们旨在确定过量铜对大鼠模型脑组织中氧化和抗氧化物质的影响。将16只雄性Wistar白化大鼠分为两组：对照组给予正常自来水，实验组给予含铜水1 g/l。4周后，在乙醚麻醉下处死所有大鼠。测定肝脏铜浓度和血浆丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）活性。多参数变化，ALT和AST活性显著升高，肝脏Cu浓度升高。测定脑组织铜浓度、超氧化物歧化酶（SOD）活性、丙二醛（MDA）水平和谷胱甘肽（GSH）浓度。过量铜处理大鼠脑铜浓度显著高于对照组（p < 0.05）。结果表明，铜中毒大鼠脑组织SOD活性和GSH水平显著低于对照组（p < 0.01和p < 0.001）。实验组脑MDA水平明显高于对照组（p < 0.001）。本研究结果表明，Cu超载引起的脂质过氧化导致脑匀浆中SOD活性和GSH水平降低，MDA水平升高。
Copper (Cu) is an integral part of many important enzymes involved in a number of vital biological processes. Even though Cu is essential to life, it can become toxic to cells, at elevated tissue concentrations. Oxidative damage due to Cu has been reported in recent studies in various tissues. In this study, we aimed to determine the effect of excess Cu on oxidative and anti-oxidative substances in brain tissue in a rat model. Sixteen male Wistar albino rats were divided into two groups: the control group, which was given normal tap water, and the experimental group, which received water containing Cu in a dose of 1 g/l. All rats were sacrificed at the end of 4 wk, under ether anesthesia. Cu concentration in the liver and in plasma alanine aminotransferase (ALT) and aspartate transaminase (AST) activities were determined. There were multiparameter changes with significant ALT and AST activity elevation and increased liver Cu concentration. In brain tissue, Cu concentration, superoxide dismutase (SOD) activities, malondialdehyde (MDA) levels and glutathione (GSH) concentrations were determined. Brain Cu concentration was significantly higher in rats receiving excess Cu, compared with control rats (p < 0.05). Our results showed that SOD activities and GSH levels in brain tissue of the Cu-intoxicated animals were significantly lower than in the control group (p < 0.01 and p < 0,001, respectively). The brain MDA levels were found to be significantly higher in the experimental group than in the control group (p < 0.001). The present results indicate that excessive Cu accumulation in the brain depressed SOD activities and GSH levels and resulted in high MDA levels in brain homogenate due to the lipid peroxidation induced by the Cu overload.