DISSOCIATION BY CYTOCHALASIN-B OF MOVEMENT, DNA-SYNTHESIS AND TRANSPORT IN 3T3 CELLS
DISSOCIATION BY CYTOCHALASIN-B OF MOVEMENT, DNA-SYNTHESIS AND TRANSPORT IN 3T3 CELLS
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DOI：
10.1002/jcp.1040850309
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发表时间：
1975-01-01
影响因子：
5.6
通讯作者：
STOKER, M
中科院分区：
文献类型：
--
作者：
BROWNSTEIN, BL;ROZENGURT, E;STOKER, M
细胞松弛素 B 被用作研究静止 3T3 细胞中细胞运动、重新启动 DNA 合成以及血清刺激的特定小分子转运增强之间相互关系的工具。浓度低于 1 μg/ml 的细胞松弛素可抑制单层内血清刺激的运动并迁移到伤口中。即使在该浓度的十倍，对培养物中 DNA 的增加也几乎没有影响，表明 DNA 合成的起始不需要远离邻近细胞。虽然 DNA 合成不会被浓度高达 10 μg/ml 的细胞松弛素抑制，但与细胞周期 S 期开始相关的胸苷转运增加受到抑制，并且不能通过用放射性胸苷标记细胞核来测量 DNA 合成。对血清添加产生的早期转运变化的影响。低浓度的药物（< 1 μg/ml）会抑制葡萄糖转运，而浓度增加 10 倍不会影响磷酸盐和尿苷的增强吸收。尽管抑制己糖摄取和细胞运动 50% 所需的细胞松弛素剂量相同，但无法证明糖转运和运动之间存在因果关系。细胞松弛素至少以两种不同的方式影响膜功能。该药物直接抑制葡萄糖的摄取，但仅影响与胸苷转运相关的 S 期增加。
Cytochalasin B was used as a tool to study the inter‐relationships between cell movement, the reinitiated DNA synthesis and the enhanced transport of specific small molecules stimulated by serum in quiescent 3T3 cells. Cytochalasin at concentrations of less than 1 μg/ml inhibits serum‐stimulated movement within the monolayer and migration into a wound. Even at ten times this concentration there is little effect on the increase in DNA in the culture, indicating that movement away from neighboring cells is not required for the initiation of DNA synthesis.While DNA synthesis is not inhibited by concentrations of cytochalasin up to 10 μg/ml, the increased thymidine transport which is associated with the onset of the S phase of the cell cycle is inhibited and DNA synthesis cannot be measured by the labelling of nuclei with radioactive thymidine.Cytochalasin has a differential effect on the early transport changes produced by serum addition. Glucose transport is inhibited by low concentrations of the drug (< 1 μg/ml) while the enhanced uptake of phosphate and uridine is unaffected by a 10‐fold increase in concentration. Although the doses of cytochalasin required for 50% inhibition of hexose uptake and of cell movement are the same, no causal relationship between sugar transport and locomotion can be demonstrated.Cytochalasin affects membrane functions in at least two different ways. The drug inhibits the uptake of glucose directly but affects only the S‐phase associated increase in thymidine transport.