Identification of Heterotrophic Nitrification in a Sierran Forest Soil
Identification of Heterotrophic Nitrification in a Sierran Forest Soil
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DOI：
10.1128/aem.48.4.802-806.1984
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发表时间：
1984-10
影响因子：
4.4
通讯作者：
J. Schimel;M. Firestone;K. Killham
中科院分区：
文献类型：
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作者：
J. Schimel;M. Firestone;K. Killham
一个潜在的异养硝化作用被确定在土壤中从成熟的针叶林和从一个明确的网站。通过使用乙炔阻断土壤泥浆中的自养NH4+氧化和氯酸盐阻断NO2−氧化为NO3 −，分别测定了NO2−产生的潜在速率和NO3−产生的潜在速率。NO2−的产生速率在森林和皆伐地的土壤中相似，并受到乙炔的强烈抑制。NO3−的生成速率远大于NO2−的生成速率，且NO3−的生成不受乙炔或氯酸盐的显著影响。放线菌酮部分抑制硝酸盐的产生，但链霉素没有显着减少。无论是添加铵还是添加蛋白胨都不能刺激NO3−的产生。NH4+池的15N标记表明NO3−不是来自NH4+。在这些森林土壤中，异养硝化作用的潜力大于自养硝化作用。
A potential for heterotrophic nitrification was identified in soil from a mature conifer forest and from a clear-cut site. Potential rates of NO2− production were determined separately from those of NO3− by using acetylene to block autotrophic NH4+ oxidation and chlorate to block NO2− oxidation to NO3− in soil slurries. Rates of NO2− production were similar in soil from the forest and the clear-cut site and were strongly inhibited by acetylene. The rate of NO3− production was much greater than that of NO2− production, and NO3− production was not significantly affected by acetylene or chlorate. Nitrate production was partially inhibited by cycloheximide, but was not significantly reduced by streptomycin. Neither the addition of ammonium nor the addition of peptone stimulated NO3− production. 15N labeling of the NH4+ pool demonstrated that NO3− was not coming from NH4+. The potential for heterotrophic nitrification in these forest soils was greater than that for autotrophic nitrification.