Integration of segmented microflow chemistry and online HPLC/MS analysis on a microfluidic chip system enabling enantioselective analyses at the nanoliter scale.
Integration of segmented microflow chemistry and online HPLC/MS analysis on a microfluidic chip system enabling enantioselective analyses at the nanoliter scale.
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DOI：
10.1039/d1lc00078k
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发表时间：
2021-05
期刊：
影响因子：
6.1
通讯作者：
S. Piendl;T. Schönfelder;Matthias Polack;Laura Weigelt;T. van der Zwaag;T. Teutenberg;E. Beckert;D. Belder
中科院分区：
文献类型：
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作者：
S. Piendl;T. Schönfelder;Matthias Polack;Laura Weigelt;T. van der Zwaag;T. Teutenberg;E. Beckert;D. Belder
在这项工作中，我们介绍了一种方法，将液滴微流体与HPLC/MS功能合并在单个芯片上，以分析单个液滴的内容。这是通过机械转子-定子接口实现的，该接口以压力密封的方式将微结构化PEEK转子精确地定位在微流体芯片上。通过选择性激光诱导蚀刻制造的开发的全身熔融石英芯片包含分段的微流室，随后是填充的HPLC通道，其通过熔融石英盖上的微流体PEEK转子与头发丝薄的通孔互连。这使得能够将小至25 nL的液滴的10 nL级分从分段微流通道靶向且无泄漏地转移到在高达60 bar的压力下操作的HPLC隔室中。在概念验证研究中，这种方法成功地应用于监测纳升规模的反应，并区分形成的对映异构体。
In this work, we introduce an approach to merge droplet microfluidics with an HPLC/MS functionality on a single chip to analyze the contents of individual droplets. This is achieved by a mechanical rotor-stator interface that precisely positions a microstructured PEEK rotor on a microfluidic chip in a pressure-tight manner. The developed full-body fused silica chip, manufactured by selective laser-induced etching, contained a segmented microflow compartment followed by a packed HPLC channel, which were interconnected by the microfluidic PEEK rotor on the fused silica lid with hair-thin through-holes. This enabled the targeted and leakage-free transfer of 10 nL fractions of droplets as small as 25 nL from the segmented microflow channel into the HPLC compartment that operated at pressures of up to 60 bar. In a proof of concept study, this approach was successfully applied to monitor reactions at the nanoliter scale and to distinguish the formed enantiomers.