The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants
The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants
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DOI：
10.1038/70781
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发表时间：
1999-12-01
影响因子：
46.9
通讯作者：
Gomez-Lim, MA
中科院分区：
文献类型：
--
作者：
Gutierrez-Campos, R;Torres-Acosta, JA;Gomez-Lim, MA
由于所有已知的多型病毒的加工机制都涉及到半胱氨酸蛋白酶的活性，我们研究了水稻半胱氨酸蛋白酶抑制剂基因的组成性表达是否能诱导转基因烟草植株对两种重要的多型病毒——烟草蚀刻病毒（TEV）和马铃薯Y病毒（PVY）产生抗性。不同水平表达外源基因的烟草株系对TEV和PVY感染的抗性进行了检测。在所有的测试品系中，稻制抑素信息水平、木瓜蛋白酶（一种半胱氨酸蛋白酶）的抑制和对TEV和PVY的抗性之间存在明确的、直接的相关性。该抑制剂对烟草花叶病毒（TMV）感染无效，因为该病毒的处理不涉及半胱氨酸蛋白酶。这些结果表明，植物半胱氨酸抑素可用于不同的多型病毒，也可能用于其他病毒，其复制涉及半胱氨酸蛋白酶活性。
As the processing mechanism of all known potyviruses involves the activity of cysteine proteinases, we asked whether constitutive expression of a rice cysteine proteinase inhibitor gene could induce resistance against two important potyviruses, tobacco etch virus (TEV) and potato virus Y (PVY), in transgenic tobacco plants. Tobacco lines expressing the foreign gene at varying levels were examined for resistance against TEV and PVY infection. There was a clear, direct correlation between the level of oryzacystatin message, inhibition of papain (a cysteine proteinase), and resistance to TEV and PVY in all lines tested. The inhibitor was ineffective against tobacco mosaic virus (TMV) infection because processing of this virus does not involve cysteine proteinases. These results show that plant cystatins can be used against different potyviruses and potentially also against other viruses, whose replication involves cysteine proteinase activity.