17β-ESTRADIOL HAS A BIPHASIC EFFECT ON GH CELL GROWTH1
17β-ESTRADIOL HAS A BIPHASIC EFFECT ON GH CELL GROWTH1
复制标题
17β-雌二醇对 GH 细胞生长具有双相效应1
DOI：
--
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
P. Dannies
中科院分区：
文献类型：
--
作者：
J. Amara;P. Dannies
雌激素在体内引起乳腺增生细胞和垂体瘤的生长，因此我们研究了雌激素对大鼠垂体瘤细胞系GH细胞生长的影响。培养基中补充了凝胶血清，使我们能够检测到雌二醇的影响，这是掩盖了较高的雌激素浓度的市售马血清。GH细胞用雌二醇处理7天。细胞数量受到影响，在一个双相的方式：雌二醇在10- 11 M和10- 11 M增加细胞数量6至13倍。3×10- 11 M和10-9 M之间的浓度导致最大细胞数呈剂量依赖性减少。这种抑制作用的半最大浓度约为10 - 10 M。催乳素的产生被雌二醇刺激3- 11倍，也具有10-10 μ M的半最大浓度。我们认为雌二醇对GH细胞至少有3种不同的作用：低于3×10 ~（-11）M的雌二醇刺激GH细胞生长，高于3 ×10 ~（-11）M的雌二醇抑制GH细胞生长。
Estrogen causes mammotroph and pituitary tumor growth i n vivo; we therefore studied its effect on growth of GH cells, a clonal line of rat pituitary tumor cells. Medium supplemented with gelding serum enabled us to detect effects of estradiol which are masked by the higher estrogen concentrations of commercially available horse serum. GH cells were treated with estradiol for 7 days. Cell number was affected in a biphasic fashion: estradiol at 10-11M and 10-11M increased cell number 6- to 13-fold. Concentrations between 3×10-11M and 10-9 M caused a dose-dependent decrease from the maximal cell number. The half maximal concentration for this inhibitory effect was about 10>-10M. Prolactin Production was stimulated 3-to 11-fold by estradiol, also with a half-maximal concentration of 10-10<M. We conclude that estradiol has at least 3 distinguishable effects on GH cells: stimulation of growth at concentration below 3×10-11M, and inhibition of growth at higher concentrations, as well as the well known stimulati...