Anaphylatoxin levels in human aqueous humor.
Anaphylatoxin levels in human aqueous humor.
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DOI：
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发表时间：
1986
影响因子：
4.4
通讯作者：
B. J. Mondino;H. Sumner
中科院分区：
文献类型：
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作者：
B. J. Mondino;H. Sumner
采用放射免疫法测定13只正常眼、8只有手术或炎症史的无炎症眼和14只前葡萄膜炎眼房水中C3a、C4a和C5a的水平。作者无法测量正常房水中C3a、C4a或C5a的水平。在有手术或炎症史的眼睛的非炎症房水中，作者无法测量C4a或C5a的水平，但在3/8的患者中能够测量低水平的C3a。在前葡萄膜炎患者的房水中，作者能够测量所有14例患者的C3a水平，9/14例患者的C4a水平，5/14例患者的C5a水平。重度前葡萄膜炎患者C3a水平高于中度前葡萄膜炎患者。与正常血浆或非炎症房水相比，炎症房水中过敏毒素与蛋白质的比例较高，这表明补体在炎症房水中通过经典途径或替代途径被激活。5例前葡萄膜炎患者C3a水平可测，C4a未检测到，提示补体激活的替代途径。
Radioimmunoassay was used to measure levels of C3a, C4a, and C5a in aqueous humor from 13 normal eyes, 8 noninflamed eyes with a history of surgery or inflammation, and 14 eyes with anterior uveitis. The authors were unable to measure levels of C3a, C4a, or C5a in normal aqueous humor. In noninflamed aqueous humor from eyes with a history of surgery or inflammation, the authors were unable to measure levels of C4a or C5a, but were able to measure low levels of C3a in 3/8 patients. In aqueous humor from eyes with anterior uveitis, the authors were able to measure levels of C3a in all 14 patients, C4a in 9/14 patients, and C5a in 5/14 patients. Patients with severe anterior uveitis had higher levels of C3a than those with moderate anterior uveitis. The higher ratios of anaphylatoxin to protein levels in inflamed aqueous humor, when compared to normal plasma or noninflamed aqueous humor, suggested that complement was being activated by either the classical or alternative pathways in inflamed aqueous humor. Measurable levels of C3a without detectable C4a in five patients with anterior uveitis suggested alternative pathway activation of complement.