Automated measurement of diameter and contraction waves of cannulated lymphatic microvessels
Automated measurement of diameter and contraction waves of cannulated lymphatic microvessels
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DOI：
10.1089/lrb.2006.4.3
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发表时间：
2006-01-01
影响因子：
1.4
通讯作者：
Gashev, Anatoliy A.
中科院分区：
文献类型：
--
作者：
Davis, Michael J.;Zawieja, David C.;Gashev, Anatoliy A.
背景：淋巴功能的研究通常采用表现出大幅度自发收缩的集合淋巴管。已经使用心脏泵类比法分析了来自这种制备的数据，所述心脏泵类比法需要精确确定血管尺寸，包括外径（OD）和内径（ID）。精确的自动测量系统将有助于这些测量。方法和结果：基于计算机的直径跟踪系统是专门为淋巴管开发的，与以前的自动化系统相比具有优势。该系统还允许在两个轴向位置进行连续直径跟踪，从而能够测量和分析收缩波传导。该方法进行了验证，有时表现出进行收缩波的大鼠胸导管自发收缩段。在这种制备中，传导波速度由轴向流速调制，并且可以容易地由跟踪系统测量。结论：该方法提供了改进，并增加了手动测量淋巴管直径的便利性，很少或没有牺牲准确性。这将是一个有用的工具，收集淋巴功能的一般研究，以及为分析收缩波的传导和协调。
Background: Studies of lymphatic function often employ collecting lymphatic vessels that exhibit large-amplitude, spontaneous contractions. Data from such preparations have been analyzed using cardiac pump analogies that require accurate determination of vascular dimensions, including external (OD) and internal (ID) diameters. These measurements would be facilitated by an accurate automated measurement system. Methods and Results: A computer based diameter tracking system was developed specifically for lymphatic vessels, with advantages over previous automated systems. The system also permits continuous diameter tracking at two axial locations, enabling the measurement and analysis of contraction wave conduction. The method was validated using spontaneously contracting segments of rat thoracic duct which sometimes exhibited conducted contraction waves. In such preparations, conduction wave velocity was modulated by the axial flow rate and could be easily measured by the tracking system. Conclusions: The method offers improvement and increased convenience over manual diameter measurements in lymphatic vessels, with little or no sacrifice in accuracy. It should be a useful tool for general studies of collecting lymphatic function as well as for the analysis of contraction wave conduction and coordination.