The differential regulation of CART gene expression in a pituitary cell line and primary cell cultures of ovine pars tuberalis cells
The differential regulation of CART gene expression in a pituitary cell line and primary cell cultures of ovine pars tuberalis cells
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DOI：
10.1046/j.1365-2826.2001.00634.x
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发表时间：
2001-04-01
影响因子：
3.2
通讯作者：
Morgan, PJ
中科院分区：
文献类型：
--
作者：
Barrett, P;Morris, MA;Morgan, PJ
可卡因-苯丙胺调节转录物（CART）编码在食欲和体重调节中具有重要作用的蛋白质。迄今为止，没有详细的分子事件和信号转导途径，调节这个基因是可用的。我们报告的鉴定CART基因表达的GH 3垂体细胞系。我们已经使用cAMP或蛋白激酶C（PKC）信号转导途径的激活剂来显示，在GH 3细胞中，CART是转录上调cAMP信号转导途径的激活剂。我们还确定了CART基因在绵羊结节部（PT）组织和原代细胞培养中的表达。与GH 3细胞相反，在PT细胞中，CART基因表达被PKC信号转导通路的激活剂上调。培养的细胞为详细分析基因转录或翻译调控、信号转导事件和许多其他细胞过程的特定调控机制提供了宝贵的资源。因此，GH 3和PT细胞可以为调节CART基因表达和加工的事件的进一步详细的分子分析提供资源。
The cocaine-amphetamine regulated transcript (CART) encodes for a protein which has an important role in the regulation of appetite and body weight. To date, no details of the molecular events and signal transduction pathways which regulate this gene are available. We report the identification of CART gene expression in the GH3 pituitary cell line. We have used activators of the cAMP or protein kinase C (PKC) signal transduction pathways to show that, in GH3 cells, CART is transcriptionally up-regulated by activators of the cAMP signal transduction pathway. We also identify CART gene expression in ovine pars tuberalis (PT) tissue and primary cell cultures. In PT cells in contrast to GH3 cells, CART gene expression is upregulated by activators of the PKC signal transduction pathway. Cultured cells have provided a valuable resource for the detailed analysis of specific regulatory mechanisms underlying transcriptional or translational regulation of genes, signal transduction events and many other cellular processes. GH3 and PT cells may therefore provide a resource for the further detailed molecular analysis of the events regulating CART gene expression and processing.