INSULIN-ANTIBODIES IN INSULIN-DEPENDENT DIABETICS BEFORE INSULIN-TREATMENT
INSULIN-ANTIBODIES IN INSULIN-DEPENDENT DIABETICS BEFORE INSULIN-TREATMENT
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DOI：
10.1126/science.6362005
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
PAQUETTE, TL
中科院分区：
文献类型：
--
作者：
PALMER, JP;ASPLIN, CM;PAQUETTE, TL
采用灵敏度高的方法测定112例新诊断的胰岛素依赖型糖尿病患者开始胰岛素治疗前血清中I-125标记胰岛素的结合率。两组非糖尿病患者作为对照组：患有糖尿病以外的各种疾病的儿童和胰岛素依赖型糖尿病患者的非糖尿病兄弟姐妹。在糖尿病患者中，18例结合率升高，36例高于对照值的第95百分位数。胰岛素结合蛋白通过抗人IgG抗体沉淀，具有与长期胰岛素依赖性糖尿病患者血清平行且在相同浓度范围内的置换曲线，并在γ球蛋白位置从Sephacryl S-300柱洗脱。这些胰岛素抗体存在于新诊断的未治疗的糖尿病患者的大部分中，并且可能是B细胞损伤的免疫标记。
A sensitive assay was used to measure the binding of I-125-labeled insulin in serum obtained from 112 newly diagnosed insulin-dependent diabetics before insulin treatment was initiated. Two groups of nondiabetics served as controls: children with a variety of diseases other than diabetes and nondiabetic siblings of insulin-dependent diabetics. Of the diabetics, 18 had elevated binding and 36 were above the 95th percentile of control values. The insulin-binding protein is precipitated by antibody to human IgG, has a displacement curve that is parallel and over the same concentration range as serum from long-standing insulin-dependent diabetics and elutes from a Sephacryl S-300 column at the position of gamma globulin. These insulin antibodies are present in a large percentage of newly diagnosed, untreated diabetics and may be an immune marker of B cell damage.