Leptomeningeal anastomosis and early ischemic lesions on diffusion-weighted imaging in male murine focal cerebral ischemia.
Leptomeningeal anastomosis and early ischemic lesions on diffusion-weighted imaging in male murine focal cerebral ischemia.
复制标题
雄性小鼠局灶性脑缺血的软脑膜吻合和弥散加权成像的早期缺血性病变。
DOI：
10.1002/jnr.24403
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Kitagawa K
中科院分区：
文献类型：
--
作者：
Saito M;Ishizuka K;Hoshino T;Toi S;Kitagawa K
软脑膜吻合术是通过弥散加权成像（DWI）确定人类中风早期缺血性病变的关键因素。然而，很少有研究在实验模型中验证这种关系。本研究试图利用小鼠模型阐明软脑膜吻合术在早期缺血性病变中的作用。对成年雄性 C57BL/6 小鼠进行单侧颈总动脉 (CCA) 闭塞或假手术。 7 天或 14 天后，大脑中动脉 (MCA) 被闭塞 45 分钟。在第一个实验中，使用磁共振成像 (MRI) DWI 对软脑膜侧支进行可视化。在第二个实验中，在 MCA 闭塞后立即进行 DWI，并在再循环后 24 小时确定梗死面积。单侧CCA闭塞缩小了早期缺血性病变的大小，扩大了软脑膜血管直径，并减轻了梗塞面积。 DWI 病变大小与软脑膜血管直径之间存在显着相关性 (r= 0.84,p< 0.01)。梗死面积与 DWI 病变大小之间的相关性也显着 (r= 0.96，p< 0.01)。总之，在小鼠模型中，早期缺血性病变中侧支循环的参与是明显的。 MRI 和软脑膜吻合术的评估均可用于开发一种针对增强侧支循环的新策略。
Leptomeningeal anastomosis is a key factor for determining early ischemic lesions on diffusion‐weighted imaging (DWI) in human stroke. However, few studies have validated this relationship in an experimental model. This study sought to clarify the involvement of leptomeningeal anastomosis in early ischemic lesions using a murine model. Adult male C57BL/6 mice were subjected to unilateral common carotid artery (CCA) occlusion or sham surgery. Seven or 14 days later, the middle cerebral artery (MCA) was occluded for 45 min. In the first experiment, the leptomeningeal collaterals were visualized using magnetic resonance imaging (MRI) DWI. In the second experiment, DWI was performed immediately after MCA occlusion, and the infarct sizes were determined 24 hr after recirculation. Unilateral CCA occlusion reduced the size of early ischemic lesions, enlarged the pial vessel diameter, and mitigated infarct size. The relationship between the DWI lesion size and pial vessel diameter was significant (r= 0.84,p< 0.01). The association between infarct size and DWI lesion size was also significant (r= 0.96,p< 0.01). In conclusion, involvement of the collateral circulation in early ischemic lesions was evident in the murine model. Both MRI and evaluation of leptomeningeal anastomosis could be used to develop a novel strategy targeting enhancement of the collateral circulation.