Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate-a surrogate of PETN, via phage display technology
Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate-a surrogate of PETN, via phage display technology
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DOI：
10.1002/bip.22997
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发表时间：
2017-03-01
期刊：
影响因子：
2.9
通讯作者：
Cortes, Pedro
中科院分区：
文献类型：
--
作者：
Kubas, George;Rees, William;Cortes, Pedro
本研究调查了选择性结合季戊四醇四硝酸酯 (PETN) 爆炸物替代品的氨基酸序列的鉴定。通过使用噬菌体展示技术和酶联免疫吸附测定 (ELISA)，对肽库进行了针对季戊四醇三硝酸酯半琥珀酸酯 (PETNH)（PETN 的替代品）的测试，以筛选那些对炸药具有亲和力的氨基酸。结果表明该文库包含对 PETNH 具有选择性的肽。经过三轮淘选后，挑选克隆并测试对 PETNH 的特异性。这些样品的 ELISA 结果表明，每个噬菌体克隆对与 PETNH 的结合具有一定程度的选择性。来自这些克隆的肽已被测序并显示其中含有某些共同的氨基酸片段。这项工作代表了一个技术平台，用于识别对任何感兴趣的生化分析物具有选择性的氨基酸序列。
The present research investigates the identification of amino acid sequences that selectively bind to a pentaerythritol tetranitrate (PETN) explosive surrogate. Through the use of a phage display technique and enzyme-linked immunosorbent assays (ELISA), a peptide library was tested against pentaerythritol trinitrate hemisuccinate (PETNH), a surrogate of PETN, to screen for those with amino acids having affinity toward the explosive. The results suggest that the library contains peptides selective to PETNH. Following three rounds of panning, clones were picked and tested for specificity toward PETNH. ELISA results from these samples show that each phage clone has some level of selectivity for binding to PETNH. The peptides from these clones have been sequenced and shown to contain certain common amino acid segments among them. This work represents a technological platform for identifying amino-acid sequences selective toward any bio-chem analyte of interest.