Development, characterization, and transferability to other Solanaceae of microsatellite markers in pepper (Capsicum annuum L.)
Development, characterization, and transferability to other Solanaceae of microsatellite markers in pepper (Capsicum annuum L.)
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DOI：
10.1139/g07-047
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发表时间：
2007-07-01
期刊：
影响因子：
3.1
通讯作者：
Ganal, Martin
中科院分区：
文献类型：
--
作者：
Nagy, Istvan;Stagel, Anik;Ganal, Martin
一组新的辣椒微卫星信息标记给出了筛选了约168 000个基因组克隆和23 174个公共数据库条目，共获得411个含微卫星的序列，可用于引物设计和功能测试。一组154个微卫星标记来源于短插入基因组文库，257个标记来源于数据库序列。在这些标记中，147个（61个来自基因组文库，86个来自数据库序列）显示出特异性和可评分的扩增产物，并检测到由栽培和野生辣椒基因型组成的测试组的33个品系中的至少2个品系之间的多态性。随后调查这些信息标记的等位基因变异和信息含量。通过构建基于微卫星多态性的系统发育树，证明了新标记在多样性和分类学研究中的实用性。通过跨物种扩增和序列比较，证明了辣椒、番茄和马铃薯中微卫星位点的一个子集的保守性。对于几个信息丰富的辣椒微卫星标记，同源表达序列标签（EST）对应物可以在这些相关的物种，也携带微卫星基序。这些直向同源物可以作为不同茄属物种遗传图谱的参考标记和共同锚定点。
A novel set of informative microsatellite markers for pepper (Capsicum annuum L.) is provided. Screening of approximately 168 000 genomic clones and 23 174 public database entries resulted in a total of 411 microsatellite-containing sequences that could be used for primer design and functional testing. A set of 154 microsatellite markers originated from short-insert genomic libraries and 257 markers originated from database sequences. Of those markers, 147 (61 from genomic libraries and 86 from database sequences) showed specific and scoreable amplification products and detected polymorphisms between at least 2 of the 33 lines of a test panel consisting of cultivated and wild Capsicum genotypes. These informative markers were subsequently surveyed for allelic variation and information content. The usefulness of the new markers for diversity and taxonomic studies was demonstrated by the construction of consistent phylogenetic trees based on the microsatellite polymorphisms. Conservation of a subset of microsatellite loci in pepper, tomato, and potato was proven by cross-species amplification and sequence comparisons. For several informative pepper microsatellite markers, homologous expressed sequence tag (EST) counterparts could be identified in these related species that also carry microsatellite motifs. Such orthologs can potentially be used as reference markers and common anchoring points on the genetic maps of different solanaccous species.