Different expression of synemin isoforms in glia and neurons during nervous system development
Different expression of synemin isoforms in glia and neurons during nervous system development
复制标题
神经系统发育过程中神经胶质和神经元中丝线蛋白亚型的不同表达
作者：
A. Izmiryan;Y. Chéraud;L. Khanamiryan;J. Leterrier;T. Federici;E. Peltékian;V. Moura;D. Paulin;Z. Li;Z. Xue
synemin基因编码属于中间丝家族的蛋白质。这些蛋白质赋予对机械应力的抵抗力并调节细胞形状。三种synemin亚型，180（H），150（M）和41（L）kDa，由前mRNA的选择性剪接产生，并在发育过程中受到不同的调节。这三种亚型的C末端尾部结构域不同，而IF杆状结构域相同。在小鼠中枢神经系统发育的早期分化过程中，Synemins H/M与巢蛋白和波形蛋白一起出现在胶质祖细胞中。它们后来在GFAP标记的细胞中被发现。相比之下，L亚型仅出现在神经元中，出生后与神经丝和βIII-微管蛋白一起出现在大脑中。然而，synemin L在周围神经系统中从E13开始出现，在那里它仅限于脊神经节的神经元。同时，synemin H/M亚型在E11的感觉神经节的神经元和雪旺细胞中被发现。从成年小鼠脊髓中分离和纯化的IFs显示，synemin L亚型结合与膜室相关的神经丝。本报告描述了合成的三个synemin异构体的选择性细胞类型，和它们的时间和空间分布。神经元和神经胶质细胞特有的机制可能控制共同synemin mRNA的剪接和每个synemin亚型的合成。© 2006 Wiley利斯公司
The synemin gene encodes proteins belonging to the intermediate filament family. These proteins confer resistance to mechanical stress and modulate cell shape. Three synemin isoforms, of 180 (H), 150 (M) and 41 (L) kDa, are produced by alternative splicing of the pre‐mRNA and are regulated differently during development. The three isoforms differ in their C‐terminal tail domains, while their IF rod domains are identical. Synemins H/M occurred together with nestin and vimentin in glial progenitors during the early differentiation of the developing mouse central nervous system. They are later found in GFAP‐labeled cells. In contrast, the L isoform appeared only in neurons, together with neurofilaments and βIII‐tubulin in the brain after birth. However, synemin L appeared from E13 in the peripheral nervous system, where it was confined to the neurons of spinal ganglia. In the meantime, the synemin H/M isoforms were found in both the neurons and Schwann cells of the sensorial ganglia from E11. Tissue fractionation and purification of IFs from adult mouse spinal cord revealed that the synemin L isoform binds to neurofilaments associated with the membrane compartment. This report describes the synthesis of the three synemin isoforms by selective cell types, and their temporal and spatial distributions. Mechanisms specific to neurons and glia probably control the splicing of the common synemin mRNA and the synthesis of each synemin isoform. © 2006 Wiley‐Liss, Inc.
神经发生过程中中间丝成分的变化。
影响因子：
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作者：
Bennett,GS
通讯作者：
Bennett,GS