Genetic characterization of porcine kobuvirus variants identified from healthy piglets in China
Genetic characterization of porcine kobuvirus variants identified from healthy piglets in China
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DOI：
10.1016/j.meegid.2015.07.035
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发表时间：
2015-10-01
影响因子：
3.2
通讯作者：
Yang, Han-Chun
中科院分区：
文献类型：
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作者：
Jin, Wen-Jie;Yang, Zhen;Yang, Han-Chun
本研究从腹泻仔猪和无症状仔猪中分别检出猪柯布病毒JS-01-CHN株和JS-02a-CHN株。利用生物信息学软件包对这两株菌株的序列进行分析。在健康仔猪中检测到JS-02a-CHN的基因组全长为8121个核苷酸(NT)，不包括Poly(A)尾巴。JS-02a-CHN的2B编码区有30个氨基酸缺失。我们首次报道了猪柯布病毒在无症状仔猪体内的30个氨基酸缺失，这表明猪柯布病毒可能根据地理和宿主的差异而进化不同。采集91头腹泻猪和126头健康猪的粪便样品，用RT-PCR方法进行检测。其中，64.8%(59/91)的腹泻仔猪和19.8%(25/126)的健康仔猪用VP1特异性引物检测到PKV。随机抽取28例病毒阳性标本进行VP1基因分析。系统进化分析表明，从腹泻猪分离的15株毒株聚为不同的分支，而来自临床健康猪的VP1序列聚为一个大群。这些结果表明，VP1基因在江苏省腹泻病猪中存在多样性，而在健康猪中则保守。(C)2015爱思唯尔B.V.保留所有权利。
In this study, two porcine kobuvirus strains, JS-01-CHN and JS-02a-CHN were detected from piglets with diarrhea and asymptomatic, respectively. The sequences of the two strains were analyzed using a bioinformatics software package. The full-length genome of JS-02a-CHN, was detected in healthy piglets was 8121 nucleotides (nt) long excluding the poly(A) tail. There was a 30 amino acid deletion in the 2B-coding region of JS-02a-CHN. We are the first to report a 30 amino acid deletion in porcine kobuvirus from asymptomatic piglets, indicating that porcine kobuvirus may have evolved differently based on geography and host differences. Fecal samples were obtained from pigs with diarrhea (n = 91) and healthy (n = 126) pigs and analyzed using RT-PCR. Of these, 64.8% (59/91) of diarrheic piglets and 19.8% (25/126) of healthy piglets were positive for PKV using VP1 specific primers. Twenty-eight (28) virus positive samples were randomly selected and the VP1 gene was analyzed. Phylogenetic analysis indicated that the 15 strains isolated from pigs with diarrhea clustered into different branches, while the VP1 sequences from clinically healthy pigs clustered into a single large group. These results indicate that the VP1 gene is diverse in pigs with diarrhea but conserved in healthy pigs in the Jiangsu Province. (C) 2015 Elsevier B.V. All rights reserved.