Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2 extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo
Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2 extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo
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DOI：
10.1016/j.jep.2011.08.055
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发表时间：
2012-06-01
影响因子：
5.4
通讯作者：
Su, Zi-Ren
中科院分区：
文献类型：
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作者：
Su, Ji-Yan;Tan, Li-Rong;Su, Zi-Ren
民族药理学相关性：Chrysanthemum indicum（Compositae）Urine，Pogostemon cablin（布兰科）Benth和Curcuma wenyujin（菊科）Y. H. Chen et C.灵是三个广泛使用的草药之间的传统中药的目的是抗炎。由上述三种中药提取物组成的中药复方CPZ具有显著的抗流感病毒活性，这与其抗炎作用密切相关。本研究的目的：研究CPZ的体内抗炎活性，为进一步探讨该复方的抗炎作用提供实验依据。通过二甲苯（DMB）诱导的小鼠耳血管扩张和乙酸诱导的毛细血管通透性增加的炎症模型，以及角叉菜胶诱导的大鼠足肿胀模型，其中通过酶联免疫吸附测定（ELISA）测定水肿爪组织中的炎症相关细胞因子，包括前列腺素E-2（PGE（2））、白细胞介素-1 β（IL-1 β）、肿瘤坏死因子-α（INF-α）和一氧化氮（NO）。结果：CPZ（340、170、85 mg/kg，小鼠，p.o.）在醋酸刺激下，不仅降低了DMB诱导的小鼠耳血管舒张，而且还减弱了毛细血管通透性。对角叉菜胶诱导的足肿胀有明显的抑制作用。ELISA结果表明，CPZ（170、85、42.5mg/kg，p.o.）能显著上调大鼠足肿胀组织中IL-1 β的水平，下调PGE（2）的水平，但对TNF-α和NO无明显影响。此外，CPZ对大鼠棉球肉芽肿的形成有一定的抑制作用，最高剂量170 mg/kg对棉球肉芽肿形成有明显的抑制作用。上述结果表明，CPZ具有较强的抗炎活性，其被指示与其对IL-1 β和PGE的调节密切相关（2）从而介导在适当水平起作用的炎症反应。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: Chrysanthemum indicum (Compositae) Urine, Pogostemon cablin (Blanco) Benth and Curcuma wenyujin (Zingiberaceae) Y. H. Chen et C. Ling are three of the extensively used herbal remedies among traditional Chinese medicines for the purpose of anti-inflammation. A traditional Chinese medicine (TCM) recipe named CPZ consisting extracts of the above three herbs, has shown noteworthy anti-influenza activity, which is closely related to its anti-inflammatory feature.Aim of this study: To investigated the anti-inflammtory activity of CPZ in vivo for a further exploration of the recipe's anti-inflammatory properties.Materials and methods: The anti-inflammatory property of CPZ on acute inflammation was evaluated by inflammatory models of dimethylbenzene (DMB)-induced ear vasodilatation and acetic acid-induced capillary permeability enhancement in mice, as well as the carrageenan-induced paw edema rat model, in which inflammation-related cytokine including prostaglandin E-2 (PGE(2)), interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (INF-alpha), and nitric oxide (NO) in the edematous paw tissue were determined by enzyme linked immunosorbent assay (ELISA). Moreover, effect of CPZ on chronic inflammation was observed through granuloma formation in rats subjected to cotton pellet implantation.Results: CPZ (340, 170, and 85 mg/kg for mice, p.o.) not only decreased the DMB-induced ear vasodilatation but also attenuated capillary permeability under acetic acid challenge in mice. And the significant inhibition on carrageenan-induced paw edema was observed. Further more, the ELISA results showed that CPZ (170, 85, and 42.5 mg/kg for rats, p.o.) could up-regulate the level of IL-1 beta in the edema paw tissue of rats significantly while down-regulate that of PGE(2), but no apparent effect on TNF-alpha or NO was observed in the test. Besides, CPZ had a certain degree of restraining effect on the cotton pellet-induced granuloma formation in rats and the highest dose of 170 mg/kg even showed a significant suppression on it.Conclusion: The above results indicated that CPZ possessed a potent anti-inflammatory activity, which is indicated to be closely associated with its regulation on IL-1 beta and PGE(2) thereby mediating the inflammatory response acting at an appropriate level. (C) 2011 Elsevier Ireland Ltd. All rights reserved.