Direct profiling of lipid distribution in brain tissue using MALDI-TOFMS
Direct profiling of lipid distribution in brain tissue using MALDI-TOFMS
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DOI：
10.1021/ac050276v
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发表时间：
2005-07-15
影响因子：
7.4
通讯作者：
Woods, AS
中科院分区：
文献类型：
--
作者：
Jackson, SN;Wang, HYJ;Woods, AS
质谱法的最新发展使直接分析组织中的生物分子成为可能。然而，大多数研究都集中在蛋白质上，强调生物标志物的发现。本文采用基质辅助激光解吸/电离质谱法对大鼠小脑脂质进行了直接分析。脂质双分子层作为小有机分子（如治疗药物）和污染物（如滴滴涕）的储存库的作用，以及比较健康和患病动物模型中脂质分布的能力，是直接探测组织以确定定性和可能的定量脂质含量可能是非常有用的工具的许多原因中的几个。以正离子模式记录胆固醇、磷脂酰胆碱、鞘磷脂、磷脂酰乙醇胺等分子离子，以负离子模式记录磷脂酰肌醇、硫脂、神经节脂苷等分子离子。总体而言，检测到具有代表性的脑脂质包括胆固醇、15种磷脂（9种磷脂酰胆碱、1种鞘磷脂、3种磷脂酰乙醇胺、2种磷脂酰肌醇）、10种硫脂（5种羟基化和5种非羟基化）和7种神经节苷类。
Recent developments in mass spectrometry have permitted direct analysis of biomolecules in tissue. However, most studies have focused on proteins with emphasis on biomarker discovery. In the present work, matrix-assisted laser desorption/ionization mass spectrometry was used for the direct analysis of lipids in rat cerebellum. The lipid bilayer role as a storage depot for small organic molecules such as therapeutic drugs and pollutants such as DDT, as well as the ability to compare lipid profiles in healthy and diseased animal models, are a few of the many reasons why the direct probing of tissue to determine the qualitative and possibly quantitative lipid content could be a very useful tool. Molecular ions corresponding to cholesterol, phosphatidylcholines, sphingomyelins, and phosphatidylethanolamines were recorded in positive ion mode, while ones corresponding to phosphatidylinositols, sulfatides, and gangliosides were recorded in negative ion mode. Overall, representatives from all major categories of brain lipids including cholesterol, 15 phospholipid species (9 phosphatidylcholines, 1 sphingomyelin, 3 phosphatidylethanolamines, 2 phosphatidylinositols), 10 sulfatides (5 hydroxylated species and 5 nonhydroxylated species), and 7 species of gangliosides were detected.