Biodegradation of poly(L-lactic acid) and poly(ε-caprolactone) patches by human amniotic fluid in an in-vitro simulated fetal environment.
Biodegradation of poly(L-lactic acid) and poly(ε-caprolactone) patches by human amniotic fluid in an in-vitro simulated fetal environment.
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DOI：
10.1038/s41598-022-07681-8
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发表时间：
2022-03-10
影响因子：
4.6
通讯作者：
Lin CY
中科院分区：
文献类型：
--
作者：
Tatu RR;Oria M;Rao MB;Peiro JL;Lin CY
开放性脊柱裂或脊髓脊膜膨出（MMC）是一种破坏性的神经系统先天性缺陷，其特征是胚胎期脊柱神经管关闭失败。由发育中的胎儿MMC脊柱缺陷引起的脑脊液泄漏可导致一系列脑异常，包括后脑疝和脑积水。挤压后的脊髓暴露于羊水中也有很大的风险，可能会因子宫内脊髓进行性损伤而导致脊髓孔下的身体部分或完全瘫痪。一项随机试验表明，通过胎儿手术进行产前修复，有时使用贴片，用水密屏障覆盖暴露的脊髓，可以有效减少产后神经系统疾病的发病率和严重程度，并减少对脑室-腹膜分流术的需求。目前，惰性或胶原基贴片的使用成本高，结构性能不足。具体来说，惰性贴片在植入后不会降解，因此需要进行与新生儿创伤相关的产后移除手术。我们目前的研究旨在对新设计的贴片进行体外降解研究，该贴片可能作为现有贴片修复MMC的优越替代方案。用聚l-乳酸和聚ε-己内酯共混制备了这种新型贴片。为期16周的羊水降解研究主要集中在跟踪结晶度和力学性能的变化。另一组设计的贴片暴露于磷酸盐缓冲盐水（PBS）中，作为时间配对对照。结晶度研究表明，在这两种介质中，膜片的水解降解都取得了进展，其中更倾向于磷酸盐缓冲盐水中的大块侵蚀和羊水中的表面侵蚀。机械测试结果表明，贴片的完整性在暴露于体液模拟物（PBS）或羊水16周内不会受到损害。
Open spina bifida or myelomeningocele (MMC) is a devastating neurologic congenital defect characterized by primary failure of neural tube closure of the spinal column during the embryologic period. Cerebrospinal fluid leak caused by the MMC spinal defect in the developing fetus can result in a constellation of encephalic anomalies that include hindbrain herniation and hydrocephalus. The exposure of extruded spinal cord to amniotic fluid also poses a significant risk for inducing partial or complete paralysis of the body parts beneath the spinal aperture by progressive spinal cord damage in-utero. A randomized trial demonstrated that prenatal repair by fetal surgery, sometimes using patches, to cover the exposed spinal cord with a watertight barrier is effective in reducing the postnatal neurologic morbidity as evidenced by decreased incidence and severity of postnatal hydrocephalus and the reduced need for ventricular-peritoneal shunting. Currently, the use of inert or collagen-based patches are associated with high costs and inadequate structural properties. Specifically, the inert patches do not degrade after implantation, causing the need for a post-natal removal surgery associated with trauma for the newborn. Our present study is aimed towards in-vitro degradation studies of a newly designed patch, which potentially can serve as a superior alternative to existing patches for MMC repair. This novel patch was fabricated by blending poly(l-lactic acid) and poly(ε-caprolactone). The 16-week degradation study in amniotic fluid was focused on tracking changes in crystallinity and mechanical properties. An additional set of designed patches was exposed to phosphate-buffered saline (PBS), as a time-paired control. Crystallinity studies indicate the progress of hydrolytic degradation of the patch in both media, with a preference to bulk erosion in phosphate buffered saline and surface erosion in amniotic fluid. Mechanical testing results establish that patch integrity is not compromised up to 16 weeks of exposure either to body fluids analog (PBS) or to amniotic fluid.
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影响因子：
5.9
作者：
Navarro-Baena, Ivan;Sessini, Valentina;Peponi, Laura
通讯作者：
Peponi, Laura
DOI：
10.1115/1.4025102
发表时间：
2013-10-01
影响因子：
1.7
作者：
Kang, Heesuk;Hollister, Scott J.;Lin, Chia-Ying
通讯作者：
Lin, Chia-Ying
DOI：
10.1590/s1679-45082015rw3032
发表时间：
2015-06-01
期刊：
Einstein (São Paulo)
影响因子：
--
作者：
Bevilacqua, Nicole Silva;Pedreira, Denise Araujo Lapa
通讯作者：
Pedreira, Denise Araujo Lapa
影响因子：
2.4
作者：
Adzick NS
通讯作者：
Adzick NS
影响因子：
--
作者：
Pina, Sandra;Ferreira, Jose M. F.
通讯作者：
Ferreira, Jose M. F.