Biodistribution of Different Sized Nanoparticles Assessed by Positron Emission Tomography: A General Strategy for Direct Activation of Metal Oxide Particles
Biodistribution of Different Sized Nanoparticles Assessed by Positron Emission Tomography: A General Strategy for Direct Activation of Metal Oxide Particles
复制标题
DOI：
10.1021/nn400450p
复制
发表时间：
2013-04-01
期刊：
影响因子：
17.1
通讯作者：
Llop, Jordi
中科院分区：
文献类型：
--
作者：
Perez-Campana, Carlos;Gomez-Vallejo, Vanessa;Llop, Jordi
NPs的体积非常小，一旦分布在材料或生物系统中，就很难检测和量化。我们提出了一种简单直接的方法，通过O-16(p, α)N-13核反应直接质子束激活合成或市购的氧化铝NPs (Al2O3 NPs)，以便使用正电子发射断层扫描（PET）评估其生物命运。经TEM、DLS和ζ电位测量证实，NPs的放射性标记不会改变其表面或结构性质。在Al2O3 NPs中掺入放射性N-13原子，可以通过PET研究静脉给药后金属氧化物NPs在大鼠体内的生物分布。尽管N-13的半衰期较短（9.97 min），但在给药后的前68 min内，可以检测到NPs在不同器官中的积累。计算每个器官的放射性百分比，以评估每个器官的NPs的相对量。这种简单而强大的激活策略可以应用于任何合成或市售的金属氧化物颗粒。
The extraordinary small size of NPs makes them difficult to detect and quantify once distributed in a material or biological system. We present a simple and straightforward method for the direct proton beam activation of synthetic or commercially available aluminum oxide NPs (Al2O3 NPs) via the O-16(p,alpha)N-13 nuclear reaction in order to assess their biological fate using positron emission tomography (PET). The radiolabeling of the NPs does not alter their surface or structural properties as demonstrated by TEM, DLS, and zeta-potential measurements. The incorporation of radioactive N-13 atoms in the Al2O3 NPs allowed the study of the biodistribution of the metal oxide NPs in rats after intravenous administration via PET. Despite the short half-life of N-13 (9.97 min), the accumulation of NPs in different organs could be measured during the first 68 min after administration. The percentage amount of radioactivity per organ was calculated to evaluate the relative amount of NPs per organ. This simple and robust activation strategy can be applied to any synthetic or commercially available metal oxide particle.