Chicken Embryonic Stem Cells: Establishment and Characterization
Chicken Embryonic Stem Cells: Establishment and Characterization
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DOI：
10.1007/978-1-62703-628-3_11
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Pain, Bertrand
中科院分区：
文献类型：
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作者：
Aubel, Pauline;Pain, Bertrand
胚胎干细胞是研究早期发育和细胞分化的独特模型。这些细胞首先在小鼠中发现，后来在其他哺乳动物中发现，也在鸟类中分离出来。在这里，使用鸡作为模型，我们描述了一组协议，允许分离，维护，遗传修饰，分化，并注射鸡胚胎干细胞（cES）到胚胎中获得嵌合动物。
Embryonic stem (ES) cells are unique models for investigating early development and cell differentiation. First identified in mouse and later in other mammals, these cells have also been isolated in avian species. Here, using chicken as a model, we describe a set of protocols allowing the isolation, maintenance, genetic modification, differentiation, and injection of the chicken embryonic stem (cES) cells into embryos for obtaining chimeric animals.