Effects of leukocyte-capillary plugging on the resistance to flow in the microvasculature of cremaster muscle for normal and activated leukocytes
Effects of leukocyte-capillary plugging on the resistance to flow in the microvasculature of cremaster muscle for normal and activated leukocytes
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DOI：
10.1006/mvre.1996.0020
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发表时间：
1996-03-01
影响因子：
3.1
通讯作者：
Lipowsky, HH
中科院分区：
文献类型：
--
作者：
Eppihimer, MJ;Lipowsky, HH
白细胞（WBC）隔离在毛细血管网络对流动阻力（R（A-V））的影响，获得在推注白细胞在提睾肌（仓鼠）。IIA-V通过同时测量小动脉到小静脉的压降和小动脉红细胞流速来计算。由于从网络中清除循环WBC，单独推注红细胞（RBC）导致R（A-V）降低5%。输注具有1至9倍全身白细胞计数的RBC导致R（A-V）短暂增加5至10%，但不显著。通过在N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）中孵育以激活（和灭活）多形核白细胞（PMN）或在佛波酯肉豆蔻酸酯（PMA）中孵育以激活推注中的所有白细胞，研究了WBC激活的效果。与正常白细胞相比，输注红细胞和活化白细胞的混合物对药物穿过毛细血管网时阻力的瞬时增加没有显著影响。然而，与正常或FMLP处理的WBC的混合物增加了稳态RA-V，与输注的WBC的累积数量成比例，这是由于大剂量冲洗后残留的毛细血管堵塞。累积输注20 × 10（6）正常或FMLP激活的WBC导致R（A-V）较基线增加25%。在PMA活化的情况下，在RBC悬液中累积输注仅5 x 10（6）个WBC也导致R（A-V）增加25%，这是相同数量的FMLP活化WBC获得的增加的三倍。累积输注12 × 10（6）PMA活化WBC后，R（A-V）异常增加至基线的约250%。PMA激活后毛细血管堵塞和RA-V的显著增加与Boli中硬化淋巴细胞（对FMLP无反应）的数量比PMNs多三倍雅阁。因此，与正常WBC相比，活化WBC引起的毛细血管堵塞可能对通过微脉管系统的血流产生更大的有害影响，并且这种影响的程度强烈依赖于循环中活化WBC的数量。(C)出版社：Academic Press，Inc.
The effects of leukocyte (WBC) sequestration in the capillary network on resistance to flow (R(A-V)) were obtained during bolus infusions of WBCs in cremaster muscle (hamster). IIA-V was calculated from simultaneous measurements of arteriole to venule pressure drop and arteriolar red cell velocity. Bolus infusions of red cells (RBCs) alone resulted in a 5% decrease in R(A-V), due to the clearance of circulating WBCs from the network. Infusions of RBCs with leukocrits of one to nine times systemic resulted in insignificant transient increases in R(A-V) of 5 to 10%. The effect of WBC activation was studied by their incubation in N-formyl-methionyl-leucyl-phenylalanine (FMLP) to activate (and stiffen) the polymorphonulear WBCs (PMNs) or phorbal myristate acetate (PMA) to activate all WBCs in the bolus. Compared with normal WBCs, infusions of mixtures of RBCs and activated WBCs had no significant effect on the transient increase in resistance as the bolus traversed the capillary network. However, mixtures with either normal or FMLP-treated WBCs increased the steady state RA-V in proportion to the cumulative number of WBCs infused, due to residual capillary plugging following washout of the bolus. The cumulative infusion of 20 x 10(6) normal or FMLP-activated WBCs resulted in a 25% increase in R(A-V) above baseline. With PMA activation, cumulative infusions of only 5 x 10(6) WBCs in the RBC suspension also resulted in a 25% increase in R(A-V), which was three times the increase obtained for an equal number of FMLP activated WBCs. Following the cumulative infusion of 12 x 10(6) PMA-activated WBCs, R(A-V) increased inordinately to approximately 250% of baseline. These substantially greater increases in capillary plugging and RA-V With PMA activation were in accord with the threefold greater number of stiffened lymphocytes (which do not respond to FMLP) relative to PMNs in the boli. Thus, capillary plugging by activated WBCs may have a far greater detrimental effect on blood flow through the microvasculature compared to normal WBCs, and the extent of this effect is strongly dependent on the number of activated WBCs in the circulation. (C) 1996 Academic Press, Inc.