Liquid matrix deposition on conductive hydrophobic surfaces for tuning and quantitation in UV-MALDI mass spectrometry
Liquid matrix deposition on conductive hydrophobic surfaces for tuning and quantitation in UV-MALDI mass spectrometry
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DOI：
10.1016/j.jasms.2006.11.013
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发表时间：
2007-04-01
影响因子：
3.2
通讯作者：
Cramer, Rainer
中科院分区：
文献类型：
--
作者：
Palmblad, Magnus;Cramer, Rainer
由于其离子产生高度波动，基质辅助激光解吸/电离 (MALDI) 为其在质谱中的应用带来了许多实际挑战。仅使用离子信号进行仪器调谐和定量离子丰度测量取决于稳定的离子束。液体 MALDI 基质已被证明是常用固体基质的有前途的替代品。已经讨论了它们在稳定离子流必不可少的领域中的应用，但仅提供了有限的数据来证明它们在形成稳定的 MALDI 离子束方面的实际用途和优势。在本文中，我们提供的实验数据显示，在 MALDI Q-TOF 质谱仪中进行定量肽丰度测量和仪器调谐时，在高分析灵敏度（低飞摩尔制备量）下，MALDI 离子束稳定性超过两个数量级。样品沉积在廉价的导电疏水表面上，并收缩至尺寸< 10 nL 的液滴。通过使用 < 10 nL 的样品液滴，可以从单个照射点采集大约 10,000 次发射的数据，在 5-20 Hz 的激光重复率下离子信号强度几乎没有变化。
With its highly fluctuating ion production matrix-assisted laser desorption/ionization (MALDI) poses many practical challenges for its application in mass spectrometry. Instrument tuning and quantitative ion abundance measurements using ion signal alone depend on a stable ion beam. Liquid MALDI matrices have been shown to be a promising alternative to the commonly used solid matrices. Their application in areas where a stable ion current is essential has been discussed but only limited data have been provided to demonstrate their practical use and advantages in the formation of stable MALDI ion beams. In this article we present experimental data showing high MALDI ion beam stability over more than two orders of magnitude at high analytical sensitivity (low femtomole amount prepared) for quantitative peptide abundance measurements and instrument tuning in a MALDI Q-TOF mass spectrometer. Samples were deposited on an inexpensive conductive hydrophobic surface and shrunk to droplets < 10 nL in size. By using a sample droplet < 10 nL it was possible to acquire data from a single irradiated spot for roughly 10,000 shots with little variation in ion signal intensity at a laser repetition rate of 5-20 Hz.