The microbiota-gut-brain interaction in regulating host metabolic adaptation to cold in male Brandt's voles (Lasiopodomys brandtii)
The microbiota-gut-brain interaction in regulating host metabolic adaptation to cold in male Brandt's voles (Lasiopodomys brandtii)
复制标题
微生物群-肠-脑相互作用调节雄性布氏田鼠（Lasiopodomysbrandtii）对寒冷的宿主代谢适应
DOI：
10.1038/s41396-019-0492-y
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发表时间：
2019-12-01
期刊：
影响因子：
11
通讯作者：
Wang, De-Hua
中科院分区：
文献类型：
--
作者：
Bo, Ting-Bei;Zhang, Xue-Ying;Wang, De-Hua
肠道微生物群在协调宿主代谢稳态中发挥着关键作用。然而，小型哺乳动物中宿主和微生物共生体之间的串扰很少被说明。我们使用雄性勃兰特田鼠（Lasiopodomysbrandtii）来测试肠道微生物群和宿主神经递质（例如去甲肾上腺素（NE））相互作用以调节冷适应过程中的能量和产热的假设。我们发现食物摄入量和生热作用的增加与冷驯化过程中单胺神经递质、生长素释放肽、短链脂肪酸和盲肠微生物群的改变有关。此外，我们的配对喂养研究表明，低温可以改变盲肠微生物群，而与过度喂养无关。使用盲肠微生物群移植以及 β3-肾上腺素受体拮抗剂和 PKA 抑制，我们证实冷适应微生物群的移植通过激活 cAMP-PKA-pCREB 信号传导增加了生热作用。此外，NE 操作还会引起肠道微生物群结构的长期改变。这些数据表明，肠道微生物群-NE通过cAMP信号传导的串扰在雄性勃兰特田鼠的冷驯化过程中调节能量和产热。
Gut microbiota play a critical role in orchestrating metabolic homeostasis of the host. However, the crosstalk between host and microbial symbionts in small mammals are rarely illustrated. We used male Brandt's voles (Lasiopodomys brandtii) to test the hypothesis that gut microbiota and host neurotransmitters, such as norepinephrine (NE), interact to regulate energetics and thermogenesis during cold acclimation. We found that increases in food intake and thermogenesis were associated with increased monoamine neurotransmitters, ghrelin, short-chain fatty acids, and altered cecal microbiota during cold acclimation. Further, our pair-fed study showed that cold temperature can alter the cecal microbiota independently of overfeeding. Using cecal microbiota transplant along with beta 3-adrenoceptor antagonism and PKA inhibition, we confirmed that transplant of cold-acclimated microbiota increased thermogenesis through activation of cAMP-PKA-pCREB signaling. In addition, NE manipulation induced a long-term alteration in gut microbiota structure. These data demonstrate that gut microbiota-NE crosstalk via cAMP signaling regulates energetics and thermogenesis during cold acclimation in male Brandt's voles.