A proteomic glimpse into human ureter proteome.
A proteomic glimpse into human ureter proteome.
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DOI：
10.1002/pmic.201500214
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发表时间：
2016-01
期刊：
影响因子：
3.4
通讯作者：
Yamamoto T
中科院分区：
文献类型：
--
作者：
Magdeldin S;Hirao Y;Elguoshy A;Xu B;Zhang Y;Fujinaka H;Yamamoto K;Yates JR 3rd;Yamamoto T
尿液由于其非侵入性采样和稳定性，已经发展成为临床蛋白质组学中最重要的生物流体之一。然而，它通过检测其组分（包括尿蛋白/多肽谱）的变化而用于多种疾病的临床诊断。尽管尿液中检测到的大多数蛋白质主要来源于泌尿生殖道（UG），但确定其在UG道内的精确来源仍然是难以捉摸的。在这篇文章中，我们对输尿管蛋白质组进行了全面的分析，以组装第一个无偏输尿管数据集。接下来，我们将这些数据与尿液、尿液外泌体和肾脏质谱数据集进行了比较。我们的结果表明，在2217个非冗余输尿管蛋白中，751个候选蛋白（33.8%）在尿液中检测到尿蛋白/多肽或外泌体蛋白。另一方面，将相应数据库中未显示的输尿管蛋白质命中（48）与膀胱和前列腺人类蛋白质图谱数据库进行比较，确定了21种可能是输尿管组织特有的蛋白质。总之，这一发现为输尿管疾病相关生物标志物（如输尿管癌）的可能鉴定提供了未来的前景。此外，本文中发表的输尿管蛋白质组数据集将为泌尿学和尿液生物标志物发现领域的研究人员提供宝贵的资源。所有MS数据均已保存在ProteomeXchange中，标识符为PXD 002620（http：//proteomecentral.proteomexchange.org/soft/PXD 002620）。
Urine has evolved as one of the most important biofluids in clinical proteomics due to its noninvasive sampling and its stability. Yet, it is used in clinical diagnostics of several disorders by detecting changes in its components including urinary protein/polypeptide profile. Despite the fact that majority of proteins detected in urine are primarily originated from the urogenital (UG) tract, determining its precise source within the UG tract remains elusive. In this article, we performed a comprehensive analysis of ureter proteome to assemble the first unbiased ureter dataset. Next, we compared these data to urine, urinary exosome, and kidney mass spectrometric datasets. Our result concluded that among 2217 nonredundant ureter proteins, 751 protein candidates (33.8%) were detected in urine as urinary protein/polypeptide or exosomal protein. On the other hand, comparing ureter protein hits (48) that are not shown in corresponding databases to urinary bladder and prostate human protein atlas databases pinpointed 21 proteins that might be unique to ureter tissue. In conclusion, this finding offers future perspectives for possible identification of ureter disease‐associated biomarkers such as ureter carcinoma. In addition, the ureter proteomic dataset published in this article will provide a valuable resource for researchers working in the field of urology and urine biomarker discovery. All MS data have been deposited in the ProteomeXchange with identifier PXD002620 (http://proteomecentral.proteomexchange.org/dataset/PXD002620).