Effect of sexual maturation on muscle gene expression of rainbow trout: RNA-Seq approach
Effect of sexual maturation on muscle gene expression of rainbow trout: RNA-Seq approach
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DOI：
10.1002/phy2.120
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发表时间：
2013-10-01
影响因子：
2.5
通讯作者：
Yao, Jianbo
中科院分区：
文献类型：
--
作者：
Salem, Mohamed;Manor, Meghan L.;Yao, Jianbo
肌肉退化是对各种生理状态的反应，这些生理状态是由特定的分子机制调节的。先前，我们描述了雌性虹鳟鱼在完全性成熟和产卵时肌肉退化的代谢变化（Salem et al., Physiol.）。基因组学2006;28: 3345;J.蛋白质组学；73: 778 - 789)。在这个模型中，肌肉退化代表了营养动员是对卵子/卵巢生长阶段能量过度需求的反应。我们最近的研究表明，肌肉生长和质量的大部分变化开始于产卵前2-3个月。与不育鱼相比，妊娠鱼表现出肌肉内脂肪减少，饱和和单不饱和脂肪酸含量较低，多不饱和脂肪酸含量较高。在这项研究中，RNA-Seq被用来解释性成熟阶段的潜在变化机制。此外，为了尽量减少由于营养不足造成的变化，鱼类被喂食高营养水平。RNA-Seq技术鉴定出与妊娠鱼代谢变化一致的基因表达特征。妊娠鱼脂肪酸降解代谢途径转录本丰度增加，不饱和脂肪酸生物合成相关基因表达上调。此外，在妊娠鱼中观察到参与柠檬酸循环和氧化磷酸化的基因表达增加。高营养水平鱼类的肌肉转录组特征与之前描述的成熟末期鱼类的肌肉转录组特征截然不同，这表明，高营养水平的雌性虹鳟鱼接近产卵时，可能动员肌肉内脂肪而不是蛋白质来支持性腺成熟。
Muscle degradation occurs as a response to various physiological states that are regulated by specific molecular mechanisms. Previously, we characterized the metabolic changes of muscle deterioration of the female rainbow trout at full sexual maturity and spawning (Salem et al., Physiol. Genomics 2006; 28: 3345; J. Proteomics 2010; 73: 778-789). Muscle deterioration in this model represents nutrient mobilization as a response to the energetic overdemands of the egg/ovarian growth phase. Our recent studies showed that most of the changes in muscle growth and quality start 2-3 months before spawning. Gravid fish exhibited reduced intramuscular fat that is lower in saturated and monounsaturated fatty acids and higher in polyunsaturated fatty acids compared to sterile fish. In this study, RNA-Seq was used to explain the mechanisms underlying changes during this phase of sexual maturity. Furthermore, to minimize changes due to nutrient deficits, fish were fed on a high-plane of nutrition. The RNA-Seq technique identified a gene expression signature that is consistent with metabolic changes of gravid fish. Gravid fish exhibited increased abundance of transcripts in metabolic pathways of fatty acid degradation and up-regulated expression of genes involved in biosynthesis of unsaturated fatty acids. In addition, increased expression of genes involved in the citric acid cycle and oxidative phosphorylation was observed for gravid fish. This muscle transcriptomic signature of fish fed on a high nutritional plane is quite distinct from that previously described for fish at terminal stages of maturity and suggest that female rainbow trout approaching spawning, on high nutritional planes, likely mobilize intramuscular fat rather than protein to support gonadal maturation.