Structural communication between the chromophore‐binding pocket and the N‐terminal extension in plant phytochrome phyB

Structural communication between the chromophore‐binding pocket and the N‐terminal extension in plant phytochrome phyB
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植物光敏色素 phyB 中发色团结合袋和 N 末端延伸之间的结构通讯

DOI:
10.1002/1873-3468.12642
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发表时间:
2017
期刊:
影响因子:
3.5
通讯作者:
Peter Hildebrandt
Peter Hildebrandt
中科院分区:
生物学3区
文献类型:
--
作者:
Francisco Velázquez Escobar;David Buhrke;Maria Fernandez Lopez;Sintayehu Shenkutie;Sylvia von Horsten;Lars-Oliver Essen;Jon Hughes;Peter Hildebrandt

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植物光敏色素的 N 端延伸(NTE)被认为在发出光诱导的结构变化信号中发挥功能作用。在这里,我们使用共振拉曼光谱来研究 NTE 对两种进化距离较远的植物的 B 型光敏色素的发色团结构的影响。 NTE缺失似乎对非活性Pr状态下的发色团没有影响,但在生理活性Pfr状态下改变了C-D环次甲基桥和周围氢键网络的扭转。这些变化伴随着两个 Pfr 亚态之间构象平衡的变化,这可能会影响 C-D 双键的热异构化速率,从而解释 NTE 对暗回复动力学的影响。
The N‐terminal extension (NTE) of plant phytochromes has been suggested to play a functional role in signaling photoinduced structural changes. Here, we use resonance Raman spectroscopy to study the effect of the NTE on the chromophore structure of B‐type phytochromes from two evolutionarily distant plants. NTE deletion seems to have no effect on the chromophore in the inactive Pr state, but alters the torsion of the C‐D ring methine bridge and the surrounding hydrogen bonding network in the physiologically active Pfr state. These changes are accompanied by a shift of the conformational equilibrium between two Pfr substates, which might affect the thermal isomerization rate of the C‐D double bond and, thus, account for the effect of the NTE on the dark reversion kinetics.
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发表时间: 2013-06-27
期刊: Cell reports
影响因子: 8.8
作者:
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