Genome-wide identification, characterization, and expression analysis of the SWEET gene family in cucumber
Genome-wide identification, characterization, and expression analysis of the SWEET gene family in cucumber
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黄瓜 SWEET 基因家族的全基因组鉴定、表征和表达分析
DOI：
10.1016/s2095-3119(16)61501-0
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发表时间：
2017-01-01
影响因子：
4.8
通讯作者：
He Hong-ju
中科院分区：
文献类型：
--
作者：
Hu Li-ping;Zhang Feng;He Hong-ju
糖转运蛋白（sugar will eventually be exported transporters）是近年来发现的一类新型糖转运蛋白，在多种生理过程中发挥重要作用。然而，植物SWEET基因家族中只有少数几个物种的功能已被确定。到目前为止，还没有对葫芦科作物的SWEET基因家族进行系统的分析。在这里，这个家庭的全基因组特性进行黄瓜（黄瓜L.）。共鉴定出17个CsSWEET基因，它们在黄瓜的7条染色体上分布不均匀。黄瓜SWEET蛋白序列具有七个保守结构域和两个推定的丝氨酸磷酸化位点。构建了黄瓜、拟南芥和水稻中SWEET基因的系统发育树，将所有SWEET基因分为4个分支。此外，一些推定的顺式元件被确定在这些CsSWEET基因的启动子区域：9种类型参与植物激素反应和8种类型参与胁迫反应。此外，CsSWEET基因的转录水平进行了分析，在各种组织中使用定量实时聚合酶链反应。大多数（70.58%）的CsSWEET基因局限于生殖组织发育。最后，鉴定出18个西瓜ClaSWEET基因和18个甜瓜CmSWEET基因，它们与CsSWEET基因具有高度的相似性。本研究结果为进一步研究CsSWEET基因在黄瓜等葫芦科作物中的功能奠定了基础。
SWEETs (sugars will eventually be exported transporters) are a novel class of recently identified sugar transporters that play important roles in diverse physiological processes. However, only a few species of the plant SWEET gene family have been functionally identified. Up till now, there has been no systematic analysis of the SWEET gene family in Cucurbitaceae crops. Here, a genome-wide characterization of this family was conducted in cucumber (Cucumis sativus L.). A total of 17 CsSWEET genes were identified, which are not evenly distributed over the seven cucumber chromosomes. Cucumber SWEET protein sequences possess seven conserved domains and two putative serine phosphorylation sites. The phylogenetic tree of the SWEET genes in cucumber, Arabidopsis thaliana, and Oryza sativa was constructed, and all the SWEET genes were divided into four clades. In addition, a number of putative cis-elements were identified in the promoter regions of these CsSWEET genes: nine types involved in phytohormone responses and eight types involved in stress responses. Moreover, the transcript levels of CsSWEET genes were analyzed in various tissues using quantitative real-time polymerase chain reaction. A majority (70.58%) of the CsSWEET genes were confined to reproductive tissue development. Finally, 18 putative watermelon ClaSWEET genes and 18 melon CmSWEET genes were identified that showed a high degree of similarity with CsSWEET genes. The results from this study provided a basic understanding of the CsSWEET genes and may also facilitate future research to elucidate the function of SWEET genes in cucumber and other Cucurbitaceae crops.