Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse.
Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse.
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发表时间：
1992-03
期刊：
影响因子：
4.6
通讯作者：
L. Niswander;G. Martin
中科院分区：
文献类型：
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作者：
L. Niswander;G. Martin
FGF -4最初作为一种转化基因从人类肿瘤中分离出来，是成纤维细胞生长因子（FGF）家族的一员。northern blot杂交分析显示，它在畸胎癌和胚胎干细胞中表达，提示它在胚胎发育中起作用。我们对发育中的小鼠胚胎（从受精到妊娠第14天（E14.5））中Fgf-4的表达进行了RNA原位杂交分析。我们的研究结果表明，Fgf-4 RNA首先在囊胚晚期的细胞中被检测到，这些细胞产生了所有的胚胎谱系（内细胞群细胞）。在原肠胚形成的早期阶段，表达仅限于形成中胚层和终胚层的原始条纹。表达继续在远端（吻侧）三分之二的条纹到大约。然后在尾芽中检测到E10，取代条纹成为中胚层的主要来源。在三个主要胚层建立和器官发生开始后，发现了其他表达位点。Fgf-4 RNA在发育中的肢芽和牙芽的鳃弓单位、体裂肌瘤、顶端外胚层脊中短暂检测到，表明该基因在胚胎发生过程中具有多重作用。这些结果与其他FGF基因的表达模式进行了比较。综上所述，这些数据表明，该基因家族的各个成员在发育途径中依次表达，如中胚层形成和肌肉形成，并在特定的上皮-间充质相互作用中发挥作用。
Fgf-4, initially isolated as a transforming gene from human tumors, is a member of the Fibroblast Growth Factor (FGF) family. It has previously been shown by northern blot hybridization analysis to be expressed in teratocarcinoma and embryonic stem cells, suggesting that it plays a role in embryonic development. We have carried out an RNA in situ hybridization analysis of Fgf-4 expression in the developing mouse embryo, from fertilization through the 14th day of gestation (E14.5). Our results show that Fgf-4 RNA is first detected at the late blastocyst stage in cells that give rise to all of the embryonic lineages (inner cell mass cells). During the early stages of gastrulation, expression becomes restricted to the primitive streak where mesoderm and definitive endoderm are formed. Expression continues in the distal (rostral) two-thirds of the streak through approx. E10, and then is detected in the tail bud, which replaces the streak as the primary source of mesoderm. Additional sites of expression are found after the three primary germ layers are established and organogenesis begins. Fgf-4 RNA is detected transiently in the branchial arch units, the somitic myotome, the apical ectodermal ridge of the developing limb bud and the tooth bud, suggesting that the gene has multiple roles during embryogenesis. These results are compared with the expression patterns of other FGF genes. Taken together, the data suggest that individual members of the gene family are expressed sequentially in developmental pathways such as mesoderm formation and myogenesis, and play a role in specific epithelial-mesenchymal interactions.